Copy Link Print Information

BNSP Antibody

(Synonyms: integrin binding sialoprotein; BSP; BNSP; SP-II; BSP-II; IBSP; Bone sialoprotein II; Cell binding sialoprotein; Integrin binding sialoprotein; SIAL_HUMAN; Bone sialoprotein 2; Cell-binding sialoprotein; Integrin-binding sialoprotein.)
Customer Review

Based on 1 Customer Validation

BNSP Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to BNSP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, ELISA

  • Reactivity :

    Mouse, Rat

  • Formulation:

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for BNSP Antibody
  • BNSP Antibody Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
You May Still Need
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-2000 1:100-500 1:100-500 1:100-500 1:5000-10000

Product Details

Description

BNSP Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to BNSP.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Mouse, Rat Predicted Reactivity: Human
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Species Reactivity Database
Immunogen

KLH conjugated synthetic peptide derived from human Bone sialoprotein II: 21-120/317

Sensitivity

Endogenous

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102014

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for BNSP Antibody
    Western blot analysis of extracts from M-Heart (lane 1) and C2C12 (lane 2) and HepG2 (lane 3) and 293T (lane 4) using BNSP antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    Binds tightly to hydroxyapatite (PubMed:11459848). Appears to form an integral part of the mineralized matrix (PubMed:1818768). Probably important to cell-matrix interaction (PubMed:1818768). Promotes adhesion and migration of various cells via the alpha-V/beta-3 integrin receptor (ITGAV:ITGB3) (PubMed:10640428, PubMed:11459848, PubMed:24103036)

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Expressed in bone (at protein level) (PubMed:11459848) . Expressed in trophoblast cells of placenta (at protein level) (PubMed:1818768) . Expressed in brain (PubMed:36261010)

  • Subunit

    Monomer (PubMed:11459848). Interacts with integrins; the interaction promotes cell adhesion (By similarity)

  • SwissProt ID

    P21815

  • Gene ID
    3381 [NCBI]
  • Synonyms

    integrin binding sialoprotein; BSP; BNSP; SP-II; BSP-II; IBSP; Bone sialoprotein II; Cell binding sialoprotein; Integrin binding sialoprotein; SIAL_HUMAN; Bone sialoprotein 2; Cell-binding sialoprotein; Integrin-binding sialoprotein.

BNSP Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from M-Heart (lane 1) and C2C12 (lane 2) and HepG2 (lane 3) and 293T (lane 4) using BNSP antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • BNSP Antibody Product Image
    1/16
  • Experimental Validation Results for BNSP Antibody
  • BNSP Antibody Product Image
  • Experimental Validation Results for BNSP Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from M-Heart (lane 1) and C2C12 (lane 2) and HepG2 (lane 3) and 293T (lane 4) using BNSP antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for BNSP Antibody
  • BNSP Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from M-Heart (lane 1) and C2C12 (lane 2) and HepG2 (lane 3) and 293T (lane 4) using BNSP antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • BNSP Antibody Product Image WB