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PEX5 Antibody (YA8286)

(Synonyms: PBD2A; PBD2B; PTS1-BP; PTS1R; PXR1; RCDP5)
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Based on 1 Customer Validation

PEX5 Antibody (YA8286) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PEX5.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    ICC/IF, FC

  • Reactivity :

    Human, Dog, Rat, Monkey, Mouse

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PEX5 Antibody (YA8286)
  • Experimental Validation Results for PEX5 Antibody (YA8286)
  • PEX5 Antibody (YA8286) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:100-250 1:100

Product Details

Description

PEX5 Antibody (YA8286) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PEX5.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Dog, Rat, Monkey, Mouse
  • Calculated Molecular Weight Predicted band size: 69.7 kDa
Immunogen

Full length human recombinant protein of human PEX5 produced in HEK293T cell.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PEX5 Antibody (YA8286)
    Immunocytochemistry analysis of HePG2 cells labeling PEX5 with PEX5 Antibody (HY-P88602) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PEX5 Antibody (HY-P88602) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PEX5 Antibody (YA8286)
    Immunocytochemistry analysis of HePG2 cells labeling PEX5 with PEX5 Antibody (HY-P88602) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PEX5 Antibody (HY-P88602) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PEX5 receptor that mediates peroxisomal import of proteins containing a C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type). Binds to cargo proteins containing a PTS1 peroxisomal targeting signal in the cytosol, and translocates them into the peroxisome matrix by passing through the PEX13-PEX14 docking complex along with cargo proteins. PEX5 receptor is then retrotranslocated into the cytosol, leading to release of bound cargo in the peroxisome matrix, and reset for a subsequent peroxisome import cycle |In addition to promoting peroxisomal translocation of proteins containing a PTS1 peroxisomal targeting signal, mediates peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal via its interaction with PEX7. Interaction with PEX7 only takes place when PEX7 is associated with cargo proteins containing a PTS2 peroxisomal targeting signal. PEX7 along with PTS2-containing cargo proteins are then translocated through the PEX13-PEX14 docking complex together with PEX5 |Does not mediate translocation of peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16].

  • Subcellular Localization

    Cytoplasm, cytosol,Peroxisome matrix

  • Expression


    Tissue_Specificity: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas

  • Isoforms & Post-Translational Modification

    P50542 has four isomers: P50542-1: 70865 Da (predicted); P50542-2: 66830 Da (predicted); P50542-3: 69872 Da (predicted); P50542-4: 72291 Da (predicted).
    Monoubiquitinated at Cys-11 by PEX2 during PEX5 passage through the retrotranslocation channel (By similarity)丨Phosphorylated at Ser-141 by ATM in response to reactive oxygen species (ROS), promoting monoubiquitination at Lys-209 and induction of pexophagy

  • Subunit

    Interacts (via WxxxF/Y and LVxEF motifs) with PEX14; promoting translocation through the PEX13-PEX14 docking complex (PubMed:11438541, PubMed:19197237, PubMed:24235149, PubMed:24662292, PubMed:28765278)

  • SwissProt ID

    P50542

  • Synonyms

    PBD2A; PBD2B; PTS1-BP; PTS1R; PXR1; RCDP5

PEX5 Antibody (YA8286) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HePG2 cells labeling PEX5 with PEX5 Antibody (HY-P88602) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PEX5 Antibody (HY-P88602) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HePG2 cells labeling PEX5 with PEX5 Antibody (HY-P88602) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PEX5 Antibody (HY-P88602) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PEX5 Antibody (YA8286) Product Image
    1/16
  • Experimental Validation Results for PEX5 Antibody (YA8286)
  • Experimental Validation Results for PEX5 Antibody (YA8286)
  • PEX5 Antibody (YA8286) Product Image
  • Experimental Validation Results for PEX5 Antibody (YA8286)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HePG2 cells labeling PEX5 with PEX5 Antibody (HY-P88602) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PEX5 Antibody (HY-P88602) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PEX5 Antibody (YA8286)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HePG2 cells labeling PEX5 with PEX5 Antibody (HY-P88602) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PEX5 Antibody (HY-P88602) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PEX5 Antibody (YA8286)
  • Experimental Validation Results for PEX5 Antibody (YA8286)
  • PEX5 Antibody (YA8286) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HePG2 cells labeling PEX5 with PEX5 Antibody (HY-P88602) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PEX5 Antibody (HY-P88602) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PEX5 Antibody (YA8286) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HePG2 cells labeling PEX5 with PEX5 Antibody (HY-P88602) at 1/200 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PEX5 Antibody (HY-P88602) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PEX5 Antibody (YA8286) Product Image ICC
  • PEX5 Antibody (YA8286) Product Image ICC