Nur77 Antibody (YA7314)

Cat. No.: HY-P87630: Data Sheet User Guide for Antibodies
FAQs
Technical Support

Nur77 Antibody (YA7314) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nur77.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Nur77 Antibody (YA7314) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nur77.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted Reactivity

Human,Mouse,Rat

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P22736

Immunogen

KLH conjugated synthetic peptide derived from human Nur77

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1000
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1000
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-1000

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*tris buffered saline (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.02% Proclin300.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Orphan nuclear receptor. Binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3' (PubMed:18690216, PubMed:8121493, PubMed:9315652). Binds 9-cis-retinoic acid outside of its ligand-binding (NR LBD) domain (PubMed:18690216). Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (PubMed:22983157). Regulates the inflammatory response in macrophages by regulating metabolic adaptations during inflammation, including repressing the transcription of genes involved in the citric acid cycle (TCA) (By similarity). Inhibits NF-kappa-B signaling by binding to low-affinity NF-kappa-B binding sites, such as at the IL2 promoter (PubMed:15466594). May act concomitantly with NR4A2 in regulating the expression of delayed-early genes during liver regeneration (By similarity). Plays a role in the vascular response to injury (By similarity)|In the cytosol, upon its detection of both bacterial lipopolysaccharide (LPS) and NBRE-containing mitochondrial DNA released by GSDMD pores during pyroptosis, it promotes non-canonical NLRP3 inflammasome activation by stimulating association of NLRP3 and NEK7

Subcellular Localization：Nucleus,Cytoplasm, cytosol,Mitochondrion

Expression：
Tissue_Specificity: Fetal muscle and adult liver, brain and thyroid
Induction: Induced by cytosporone B (Csn-B); directly stimulates its own expression (PubMed:18690216). Induced by corticotropin-releasing hormone (CRH) (PubMed:9315652). Induced by growth-stimulating agents (PubMed:1651101)

Isoforms & Post-Translational Modification：P22736 has three isomers: P22736-1: 64463 Da (predicted); P22736-2: 65998 Da (predicted); P22736-3: 33754 Da (predicted).
Phosphorylated at Ser-351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli丨Acetylated by p300/CBP, acetylation increases stability

Subunit：Binds the NGFI-B response element (NBRE) as a monomer (PubMed:18690216)

Documentation

User Guide for Antibodies (1077 KB)