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C-erbB-2/HER2 Antibody (YA388)

Customer Review

Based on 1 Customer Validation

C-erbB-2/HER2 Antibody (YA388) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to C-erbB-2/HER2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC, mIHC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for C-erbB-2/HER2 Antibody (YA388)
  • Experimental Validation Results for C-erbB-2/HER2 Antibody (YA388)
  • Experimental Validation Results for C-erbB-2/HER2 Antibody (YA388)
  • C-erbB-2/HER2 Antibody (YA388) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
mIHC Info
mIHC: Multiplex Immunohistochemical
Dilution Ratio 1:2000 1:1000 1:50 1:1000 1:2000

Product Details

Description

C-erbB-2/HER2 Antibody (YA388) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to C-erbB-2/HER2.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 250 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 138 kDa
Species Reactivity Database

Entrez Gene: 2064 Human

SwissProt: P04626 Human

Immunogen

Synthetic peptide corresponding to Human ErbB2/ HER2.AA range:500-650.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102573

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for C-erbB-2/HER2 Antibody (YA388)
    Western blot analysis of extracts from A549(lane2(20μg) and A549(lane 3(40μg) using HER2 Antibody (HY-P80500) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for C-erbB-2/HER2 Antibody (YA388)
    Immunocytochemistry analysis of MCF-7 cells labeling HER2 with HER2 Antibody (HY-P80500) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HER2 Antibody (HY-P80500) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for C-erbB-2/HER2 Antibody (YA388)
    Immunocytochemistry analysis of Hela cells labeling HER2 with HER2 Antibody (HY-P80500) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HER2 Antibody (HY-P80500)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization; In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell projection, ruffle membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Early endosome; Cytoplasm, perinuclear region; Nucleus; Cytoplasm; Nucleus; Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:This gene is expressed in a variety of tumor tissues, including primary breast tumors as well as tumors of the small intestine, esophagus, kidneys, and oral cavity.

  • Isoforms & Post-Translational Modification

    P04626 has 6 isomers: P04626-1: 137910 Da (predicted); P04626-2: 70917 Da (predicted); P04626-3: 62568 Da (predicted); P04626-4: 136501 Da (predicted); P04626-5: 134856 Da (predicted); P04626-6: 97382 Da (predicted).
    Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172, PubMed:33497358). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172)

  • Subunit

    Homodimer (PubMed:21454582). Heterodimer with EGFR, ERBB3 and ERBB4 (PubMed:10358079, PubMed:15093539, PubMed:16978839, PubMed:21190959).

  • SwissProt ID

    P04626

  • Gene ID
    2064 [NCBI]
  • Research Field

    Tags & Cell Markers

C-erbB-2/HER2 Antibody (YA388) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane2(20μg) and A549(lane 3(40μg) using HER2 Antibody (HY-P80500) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling HER2 with HER2 Antibody (HY-P80500) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HER2 Antibody (HY-P80500) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling HER2 with HER2 Antibody (HY-P80500) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HER2 Antibody (HY-P80500)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • C-erbB-2/HER2 Antibody (YA388) Product Image
    1/16
  • Experimental Validation Results for C-erbB-2/HER2 Antibody (YA388)
  • Experimental Validation Results for C-erbB-2/HER2 Antibody (YA388)
  • Experimental Validation Results for C-erbB-2/HER2 Antibody (YA388)
  • C-erbB-2/HER2 Antibody (YA388) Product Image
  • Experimental Validation Results for C-erbB-2/HER2 Antibody (YA388)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane2(20μg) and A549(lane 3(40μg) using HER2 Antibody (HY-P80500) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for C-erbB-2/HER2 Antibody (YA388)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling HER2 with HER2 Antibody (HY-P80500) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HER2 Antibody (HY-P80500) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for C-erbB-2/HER2 Antibody (YA388)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling HER2 with HER2 Antibody (HY-P80500) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HER2 Antibody (HY-P80500)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for C-erbB-2/HER2 Antibody (YA388)
  • Experimental Validation Results for C-erbB-2/HER2 Antibody (YA388)
  • Experimental Validation Results for C-erbB-2/HER2 Antibody (YA388)
  • C-erbB-2/HER2 Antibody (YA388) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549(lane2(20μg) and A549(lane 3(40μg) using HER2 Antibody (HY-P80500) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • C-erbB-2/HER2 Antibody (YA388) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling HER2 with HER2 Antibody (HY-P80500) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HER2 Antibody (HY-P80500) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • C-erbB-2/HER2 Antibody (YA388) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling HER2 with HER2 Antibody (HY-P80500) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HER2 Antibody (HY-P80500)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • C-erbB-2/HER2 Antibody (YA388) Product Image WB
  • C-erbB-2/HER2 Antibody (YA388) Product Image ICC
  • C-erbB-2/HER2 Antibody (YA388) Product Image ICC