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Erlin-2 Antibody (YA6565)

(Synonyms: C8orf2 antibody; Chromosome 8 open reading frame 2 antibody; Endoplasmic reticulum lipid raft associated protein 2 antibody; Endoplasmic reticulum lipid raft-associated protein 2 antibody; ER lipid raft associated 2 antibody; ERLIN 2 antibody; Erlin-2 antibody; ERLIN2 antibody; ERLN2_HUMAN antibody; HGNC:1356 antibody; C8orf2 antibody; Chromosome 8 open reading frame 2 antibody; Endoplasmic reticulum lipid raft associated protein 2 antibody; Endoplasmic reticulum lipid raft-associated protein 2 antibody; ER lipid raft associated 2 antibody; ERLIN 2 antibody; Erlin-2 antibody; ERLIN2 antibody; ERLN2_HUMAN antibody; HGNC:1356 antibody; MGC87072 antibody; NET32 antibody; SPFH 2 antibody; SPFH domain containing protein 2 antibody; SPFH domain family member 2 antibody; SPFH domain-containing protein 2 antibody; SPFH2 antibody; SPG18 antibody; Stomatin prohibitin flotillin HflC/K domain containing protein 2 antibody; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2 antibody; )
Customer Review

Based on 1 Customer Validation

Erlin-2 Antibody (YA6565) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Erlin-2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, FC, IF-Tissue

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Erlin-2 Antibody (YA6565) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-2000 1:100-500 1:100-500 1:50-200 1:100-500 1:50-100

Product Details

Description

Erlin-2 Antibody (YA6565) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Erlin-2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 38 kDa
Immunogen

Recombinant protein within human Erlin-2: 81-118.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 1), HeLa (lane 2), A549 (lane 3), Mouse brain (lane 4), and Mouse kidney (lane 5) using Erlin-2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    Immunohistochemical analysis of paraffin-embedded human glioma using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    Immunohistochemical analysis of paraffin-embedded human lymph node using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    Immunohistochemical analysis of paraffin-embedded human head and neck cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    Immunohistochemical analysis of paraffin-embedded human placenta using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    Immunohistochemical analysis of paraffin-embedded human breast cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Erlin-2 component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1. Promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex. Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. May promote ER retention of the SCAP-SREBF complex[1][2][3][4].

  • Subcellular Localization

    Endoplasmic reticulum membrane

  • Expression


    Tissue_Specificity: Ubiquitous

  • Isoforms & Post-Translational Modification

    O94905 has three isomers: O94905-1: 37840 Da (predicted); O94905-2: 16814 Da (predicted); O94905-3: 22939 Da (predicted).
    Deubiquitinated by USP25; leading to stabilization

  • Subunit

    Forms a heteromeric complex with ERLIN1

  • SwissProt ID

    O94905

  • Gene ID
    11160 [NCBI]
  • Synonyms

    C8orf2 antibody; Chromosome 8 open reading frame 2 antibody; Endoplasmic reticulum lipid raft associated protein 2 antibody; Endoplasmic reticulum lipid raft-associated protein 2 antibody; ER lipid raft associated 2 antibody; ERLIN 2 antibody; Erlin-2 antibody; ERLIN2 antibody; ERLN2_HUMAN antibody; HGNC:1356 antibody; C8orf2 antibody; Chromosome 8 open reading frame 2 antibody; Endoplasmic reticulum lipid raft associated protein 2 antibody; Endoplasmic reticulum lipid raft-associated protein 2 antibody; ER lipid raft associated 2 antibody; ERLIN 2 antibody; Erlin-2 antibody; ERLIN2 antibody; ERLN2_HUMAN antibody; HGNC:1356 antibody; MGC87072 antibody; NET32 antibody; SPFH 2 antibody; SPFH domain containing protein 2 antibody; SPFH domain family member 2 antibody; SPFH domain-containing protein 2 antibody; SPFH2 antibody; SPG18 antibody; Stomatin prohibitin flotillin HflC/K domain containing protein 2 antibody; Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2 antibody;

Erlin-2 Antibody (YA6565) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 1), HeLa (lane 2), A549 (lane 3), Mouse brain (lane 4), and Mouse kidney (lane 5) using Erlin-2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human glioma using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human head and neck cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Erlin-2 Antibody (YA6565) Product Image
    1/16
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Erlin-2 Antibody (YA6565) Product Image
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 1), HeLa (lane 2), A549 (lane 3), Mouse brain (lane 4), and Mouse kidney (lane 5) using Erlin-2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human glioma using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreatic cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human head and neck cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Experimental Validation Results for Erlin-2 Antibody (YA6565)
  • Erlin-2 Antibody (YA6565) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 1), HeLa (lane 2), A549 (lane 3), Mouse brain (lane 4), and Mouse kidney (lane 5) using Erlin-2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Erlin-2 Antibody (YA6565) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human glioma using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Erlin-2 Antibody (YA6565) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lymph node using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Erlin-2 Antibody (YA6565) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Erlin-2 Antibody (YA6565) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human head and neck cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Erlin-2 Antibody (YA6565) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Erlin-2 Antibody (YA6565) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Erlin-2 Antibody (YA6565) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human non-small cell lung cancer using Erlin-2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P86872, 1/500) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Erlin-2 Antibody (YA6565) Product Image WB
  • Erlin-2 Antibody (YA6565) Product Image IHC-P
  • Erlin-2 Antibody (YA6565) Product Image IHC-P
  • Erlin-2 Antibody (YA6565) Product Image IHC-P
  • Erlin-2 Antibody (YA6565) Product Image IHC-P
  • Erlin-2 Antibody (YA6565) Product Image IHC-P
  • Erlin-2 Antibody (YA6565) Product Image IHC-P
  • Erlin-2 Antibody (YA6565) Product Image IHC-P