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WLS/GPR177 Antibody (YA7000)

(別名: C1orf139 antibody; DKFZp686I0788 antibody; Evenness interrupted, Drosophila, homolg of antibody; EVI antibody; FLJ23091 antibody; G protein coupled receptor 177 antibody; GPR 177 antibody; Integral membrane protein GPR177 antibody; MGC131760 antibody; MGC14878 antibody)
1 Cited Publications
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Based on 1 publication(s) in Google Scholar

WLS/GPR177 Antibody (YA7000) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to WLS/GPR177.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for WLS/GPR177 Antibody (YA7000)
  • Experimental Validation Results for WLS/GPR177 Antibody (YA7000)
  • Experimental Validation Results for WLS/GPR177 Antibody (YA7000)
  • WLS/GPR177 Antibody (YA7000) Product Image
ALL WB ICC
  • Experimental Validation Results for WLS/GPR177 Antibody (YA7000)
  • Experimental Validation Results for WLS/GPR177 Antibody (YA7000)
  • Experimental Validation Results for WLS/GPR177 Antibody (YA7000)
  • WLS/GPR177 Antibody (YA7000) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

MedChemExpress（MCE）の使用を引用している文献 WLS/GPR177 Antibody (YA7000)

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:5000-1:20000 1:100

Product Details

製品説明

WLS/GPR177 Antibody (YA7000) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to WLS/GPR177.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Immunogen

Recombinant protein within human WLS aa 50-200/541.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2b

RRID

AB_3719304

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • 輸送条件

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for WLS/GPR177 Antibody (YA7000)
    Western blot analysis of extracts from Hela (lane 2(20μg)
  • Experimental Validation Results for WLS/GPR177 Antibody (YA7000)
    Immunocytochemistry analysis of HeLa cells labeling WLS/GPR177 with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for WLS/GPR177 Antibody (YA7000)
    Immunocytochemistry analysis of SH-SY5Y cells labeling WLS/GPR177 with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins (PubMed:34587386). Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity)

  • Subcellular Localization

    Golgi apparatus membrane,Cytoplasmic vesicle membrane,Cell membrane,Endoplasmic reticulum membrane,Golgi apparatus membrane,Early endosome membrane

  • Isoforms & Post-Translational Modification

    Q5T9L3 has three isomers: Q5T9L3-1: 62253 Da (predicted); Q5T9L3-2: 62552 Da (predicted); Q5T9L3-3: 51571 Da (predicted).
    N-glycosylated

  • Subunit

    Interacts with WNT3A

  • SwissProt ID

    Q5T9L3

  • 別名

    C1orf139 antibody; DKFZp686I0788 antibody; Evenness interrupted, Drosophila, homolg of antibody; EVI antibody; FLJ23091 antibody; G protein coupled receptor 177 antibody; GPR 177 antibody; Integral membrane protein GPR177 antibody; MGC131760 antibody; MGC14878 antibody

WLS/GPR177 Antibody (YA7000) 関連分類

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    WLS/GPR177 Antibody (YA7000) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg)

  • 1/16

    WLS/GPR177 Antibody (YA7000) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling WLS/GPR177 with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    WLS/GPR177 Antibody (YA7000) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling WLS/GPR177 with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • WLS/GPR177 Antibody (YA7000) Product Image
    1/16
  • Experimental Validation Results for WLS/GPR177 Antibody (YA7000)
  • Experimental Validation Results for WLS/GPR177 Antibody (YA7000)
  • Experimental Validation Results for WLS/GPR177 Antibody (YA7000)
  • WLS/GPR177 Antibody (YA7000) Product Image
  • WLS/GPR177 Antibody (YA7000) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg)

  • WLS/GPR177 Antibody (YA7000) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling WLS/GPR177 with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • WLS/GPR177 Antibody (YA7000) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of SH-SY5Y cells labeling WLS/GPR177 with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • WLS/GPR177 Antibody (YA7000) Product Image WB
  • WLS/GPR177 Antibody (YA7000) Product Image ICC
  • WLS/GPR177 Antibody (YA7000) Product Image ICC