WLS/GPR177 Antibody (YA7000)

Cat. No.: HY-P87317: Data Sheet COA
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WLS/GPR177 Antibody (YA7000) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to WLS/GPR177.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

WLS/GPR177 Antibody (YA7000) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to WLS/GPR177.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 62 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q5T9L3

Immunogen

Recombinant protein within human WLS aa 50-200/541.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000-1:20000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from Hela (lane 2(20μg)
Immunocytochemistry analysis of HeLa cells labeling WLS/GPR177 with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of SH-SY5Y cells labeling WLS/GPR177 with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with WLS/GPR177 Antibody (HY-P87317) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins (PubMed:34587386). Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity)

Subcellular Localization：Golgi apparatus membrane,Cytoplasmic vesicle membrane,Cell membrane,Endoplasmic reticulum membrane,Golgi apparatus membrane,Early endosome membrane

Isoforms & Post-Translational Modification：Q5T9L3 has three isomers: Q5T9L3-1: 62253 Da (predicted); Q5T9L3-2: 62552 Da (predicted); Q5T9L3-3: 51571 Da (predicted).
N-glycosylated

Subunit：Interacts with WNT3A

RRID

AB_3719304

Synonyms

C1orf139 antibody; DKFZp686I0788 antibody; Evenness interrupted, Drosophila, homolg of antibody; EVI antibody; FLJ23091 antibody; G protein coupled receptor 177 antibody; GPR 177 antibody; Integral membrane protein GPR177 antibody; MGC131760 antibody; MGC14878 antibody

Documentation

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)