Hck Antibody (YA2963)(PBS only)
(Synonyms: HCK; Tyrosine-protein kinase HCK; Hematopoietic cell kinase; Hemopoietic cell kinase; p59-HCK/p60-HCK; p59Hck; p61Hck)Hck Antibody (YA2963) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Hck.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IP
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:200 | 1:20 |
Product Details
Hck Antibody (YA2963) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Hck.
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Host Rabbit
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 60 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 60 kDa
A synthetic peptide of human Hck
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Hck, a member of the Src family non-receptor tyrosine kinases, functions as a critical regulator of intracellular signaling, modulating protein tyrosine phosphorylation and downstream effector activation[1][2]. Mechanistically, Hck participates in immune cell migration and monocyte chemotaxis through Src-MAPK and ERK1/2 signaling pathways, integrating extracellular adhesion cues into cytoskeletal reorganization[2]. In disease models, Hck activity contributes to inflammatory processes in rheumatoid arthritis and atherosclerosis, and is implicated in malignancies through dysregulated Src family kinase signaling[1][2]. Compared with related isoforms, such as Src and Lyn, Hck exhibits selective expression in hematopoietic lineages and unique responsiveness to chemotactic and adhesion stimuli, enabling differential regulation of monocyte versus general leukocyte signaling[3][2]. Pharmacologically, small-molecule inhibitors targeting Hck’s inactive conformation demonstrate selective suppression of Hck-mediated signaling without affecting closely related kinases, providing tools for dissecting its role in immune and cancer models[1]. These inhibitors exploit structural features of Hck to reduce promiscuity and cytotoxicity, supporting their application in both experimental and potential therapeutic contexts[1]. Collectively, these findings establish Hck as a functionally distinct kinase within the Src family, mediating critical signaling in immunity and disease, and amenable to isoform-specific pharmacological intervention[1][2].
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Subcellular Localization
Lysosome; Membrane; Lipid-anchor; Cell projection, podosome membrane; Lipid-anchor; Cytoplasm, cytosol; Cell membrane; Lipid-anchor; Membrane, caveola; Lipid-anchor; Cell junction, focal adhesion; Cytoplasm, cytoskeleton; Golgi apparatus; Cytoplasmic vesicle; Lysosome; Nucleus; Cytoplasmic vesicle, secretory vesicle; Cytoplasm, cytosol
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Expression
Tissue_specificity:Detected in monocytes and neutrophils (protein levels) . Primarily expressed in myeloid and B lymphoid cells. Highly expressed in granulocytes. Detected in tonsils.
Induction:Up-regulated during myeloid cell differentiation. The highest levels are detected in fully differentiated phagocytes. Up-regulated by IL2 -
Isoforms & Post-Translational Modification
P08631 has 4 isomers: P08631-1: 59600 Da (predicted); P08631-2: 57312 Da (predicted); P08631-3: 57241 Da (predicted); P08631-4: 59529 Da (predicted).
Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation by the BCR-ABL fusion protein mediates activation of HCK. Phosphorylation at Tyr-411 increases kinase activity. Phosphorylation at Tyr-522 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-522, suggesting that this site is a target of other kinases;Ubiquitinated by CBL, leading to its degradation via the proteasome;Isoform 2 palmitoylation at position 2 requires prior myristoylation. Palmitoylation at position 3 is required for caveolar localization of isoform 2 -
Subunit
Interacts (via SH2 domain) with FLT3 (tyrosine phosphorylated). Interacts with VAV1, WAS and RAPGEF1 (By similarity). This interaction stimulates its tyrosine-kinase activity. Interacts with ARRB1 and ARRB2. Interacts with ADAM15. Interacts with FASLG. Interacts with CBL. Interacts with FCGR1A; the interaction may be indirect. Interacts with IL6ST. Interacts (via SH3 domain) with ELMO1. Interacts (via SH3 domain) with TP73. Interacts with YAP1. Interacts with ABL1 and ITGB1, and thereby recruits ABL1 to activated ITGB1. Interacts (via SH3 domain) with WDCP
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SwissProt ID
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Synonyms
HCK; Tyrosine-protein kinase HCK; Hematopoietic cell kinase; Hemopoietic cell kinase; p59-HCK/p60-HCK; p59Hck; p61Hck
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Research Field
Signal Transduction
Documentation
References
[1]. Chakraborty MP, et al. Selective targeting of the inactive state of hematopoietic cell kinase (Hck) with a stable curcumin derivative. J Biol Chem. 2021 Jan-Jun;296:100449. [Content Brief]
[2]. Lalancette C, et al. Bull testicular haploid germ cells express a messenger encoding for a truncated form of the protein tyrosine kinase HCK. Mol Reprod Dev. 2006 Apr;73(4):520-30. [Content Brief]
[3]. Kumar P, et al. Soluble E-selectin induces monocyte chemotaxis through Src family tyrosine kinases. J Biol Chem. 2001 Jun 15;276(24):21039-45. [Content Brief]
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