CFLAR Antibody (YA3531)(PBS only)
(Synonyms: CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1)CFLAR Antibody (YA3531) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CFLAR.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P, FC, ELISA
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
CFLAR Antibody (YA3531) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CFLAR.
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Host Mouse
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 55 kDa
Purified recombinant fragment of human CFLAR (AA: 100-251) expressed in E. Coli.
affinity purified.
Non-conjugated
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CFLAR is an Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity
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Expression
Tissue_specificity:Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle
Induction:Repressed by IL2/interleukin-2 after TCR stimulation, during progression to the S phase of the cell cycle -
Isoforms & Post-Translational Modification
O15519 has 15 isomers: O15519-1: 55344 Da (predicted); O15519-2: 25379 Da (predicted); O15519-3: 41319 Da (predicted); O15519-4: 27055 Da (predicted); O15519-5: 51390 Da (predicted); O15519-6: 50661 Da (predicted); O15519-7: 39245 Da (predicted); O15519-8: 51330 Da (predicted); O15519-9: 30399 Da (predicted); O15519-10: 34384 Da (predicted); O15519-11: 52551 Da (predicted); O15519-12: 33273 Da (predicted); O15519-13: 23289 Da (predicted); O15519-14: 49778 Da (predicted); O15519-15: 44112 Da (predicted).
Proteolytically processed by CASP8 generating subunit p43 and p12 -
Subunit
TNFRSF6 stimulation triggers recruitment to the death-inducing signaling complex (DISC) formed by TNFRSF6, FADD and CASP8 (PubMed:9880531). A proteolytic fragment (p43) stays associated with the DISC (PubMed:9880531). Also interacts with FADD, CASP8, CASP3, TRAF1, TRAF2 and Bcl-X(L) (in vitro) (PubMed:9208847, PubMed:9326610). Interacts with RIPK1 (By similarity) (PubMed:9208847, PubMed:9326610, PubMed:9880531)
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SwissProt ID
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Synonyms
CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
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