CFLAR Antibody (YA3531)(PBS only)

Cat. No.: HY-P83834A: Data Sheet User Guide for Antibodies
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CFLAR Antibody (YA3531) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CFLAR.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CFLAR Antibody (YA3531) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CFLAR.

Host

Mouse

Molecular Weight

Predicted band size: 55 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

O15519

Gene ID

8837 [NCBI]

Immunogen

Purified recombinant fragment of human CFLAR (AA: 100-251) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity

Expression：
Tissue_specificity:Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle

Induction:Repressed by IL2/interleukin-2 after TCR stimulation, during progression to the S phase of the cell cycle

Isoforms & Post-Translational Modification：O15519 has 15 isomers: O15519-1: 55344 Da (predicted); O15519-2: 25379 Da (predicted); O15519-3: 41319 Da (predicted); O15519-4: 27055 Da (predicted); O15519-5: 51390 Da (predicted); O15519-6: 50661 Da (predicted); O15519-7: 39245 Da (predicted); O15519-8: 51330 Da (predicted); O15519-9: 30399 Da (predicted); O15519-10: 34384 Da (predicted); O15519-11: 52551 Da (predicted); O15519-12: 33273 Da (predicted); O15519-13: 23289 Da (predicted); O15519-14: 49778 Da (predicted); O15519-15: 44112 Da (predicted).
Proteolytically processed by CASP8 generating subunit p43 and p12

Subunit：TNFRSF6 stimulation triggers recruitment to the death-inducing signaling complex (DISC) formed by TNFRSF6, FADD and CASP8 (PubMed:9880531). A proteolytic fragment (p43) stays associated with the DISC (PubMed:9880531). Also interacts with FADD, CASP8, CASP3, TRAF1, TRAF2 and Bcl-X(L) (in vitro) (PubMed:9208847, PubMed:9326610). Interacts with RIPK1 (By similarity) (PubMed:9208847, PubMed:9326610, PubMed:9880531)

Synonyms

CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1

Documentation

User Guide for Antibodies (1077 KB)