ERCC8 Antibody (YA2901)(PBS only)
(Synonyms: CKN1; CSA; ERCC8)ERCC8 Antibody (YA2901) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERCC8.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IP
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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|---|---|---|
| Dilution Ratio | 1:1000-1:5000 | 1:10-1:100 |
Product Details
ERCC8 Antibody (YA2901) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERCC8.
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Host Rabbit
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 44 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 44 kDa
A synthetic peptide of human ERCC8
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ERCC8 is a Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair (TC-NER), a process during which RNA polymerase II-blocking lesions are rapidly removed from the transcribed strand of active genes. Following recruitment to lesion-stalled RNA polymerase II (Pol II), the CSA complex mediates ubiquitination of Pol II subunit POLR2A/RPB1 at 'Lys-1268', a critical TC-NER checkpoint, governing RNA Pol II stability and initiating DNA damage excision by TFIIH recruitment. The CSA complex also promotes the ubiquitination and subsequent proteasomal degradation of ERCC6/CSB in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. Also plays a role in DNA double-strand breaks (DSSBs) repair by non-homologous end joining (NHEJ)[1][2][3][4][5][6][7][8][9][10].
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Subcellular Localization
Nucleus; Chromosome; Nucleus matrix
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Isoforms & Post-Translational Modification
Q13216 has 2 isomers: Q13216-1: 44055 Da (predicted); Q13216-2: 23182 Da (predicted).
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Subunit
Part of the CSA complex (also named DCX(ERCC8) complex), a DCX E3 ubiquitin-protein ligase complex containing ERCC8, RBX1, DDB1 and CUL4A; the CSA complex interacts with RNA polymerase II; upon UV irradiation it interacts with the COP9 signalosome and preferentially with the hyperphosphorylated form of RNA polymerase II (PubMed:12732143, PubMed:22118460, PubMed:32355176, PubMed:34526721, PubMed:38316879). Interacts with ERCC6/CSB (via CIM motif); promoting recruitment to lesion-stalled RNA polymerase II (Pol II) (PubMed:16751180, PubMed:32355176, PubMed:7664335). Interacts with KIAA1530/UVSSA (PubMed:22466612). Interacts with a subunit of RNA polymerase II TFIIH (PubMed:7664335)
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SwissProt ID
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Synonyms
CKN1; CSA; ERCC8
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Research Field
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Documentation
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