ERCC8 Antibody (YA2901)(PBS only)

Cat. No.: HY-P83156A: Data Sheet User Guide for Antibodies
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ERCC8 Antibody (YA2901) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERCC8.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ERCC8 Antibody (YA2901) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERCC8.

Host

Rabbit

Molecular Weight

Predicted band size: 44 kDa;

Observed band size: 44 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q13216

Gene ID

1161 [NCBI]

Immunogen

A synthetic peptide of human ERCC8

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IP IP: Immunoprecipitation	1:10-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair (TC-NER), a process during which RNA polymerase II-blocking lesions are rapidly removed from the transcribed strand of active genes (PubMed:12732143, PubMed:16751180, PubMed:16964240, PubMed:32142649, PubMed:34526721, PubMed:38316879, PubMed:38600235, PubMed:38600236). Following recruitment to lesion-stalled RNA polymerase II (Pol II), the CSA complex mediates ubiquitination of Pol II subunit POLR2A/RPB1 at 'Lys-1268', a critical TC-NER checkpoint, governing RNA Pol II stability and initiating DNA damage excision by TFIIH recruitment (PubMed:12732143, PubMed:16751180, PubMed:16964240, PubMed:32142649, PubMed:32355176, PubMed:34526721, PubMed:38316879, PubMed:38600235, PubMed:38600236). The CSA complex also promotes the ubiquitination and subsequent proteasomal degradation of ERCC6/CSB in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair (PubMed:16751180). Also plays a role in DNA double-strand breaks (DSSBs) repair by non-homologous end joining (NHEJ) (PubMed:29545921)

Subcellular Localization：Nucleus; Chromosome; Nucleus matrix

Isoforms & Post-Translational Modification：Q13216 has 2 isomers: Q13216-1: 44055 Da (predicted); Q13216-2: 23182 Da (predicted).

Subunit：Part of the CSA complex (also named DCX(ERCC8) complex), a DCX E3 ubiquitin-protein ligase complex containing ERCC8, RBX1, DDB1 and CUL4A; the CSA complex interacts with RNA polymerase II; upon UV irradiation it interacts with the COP9 signalosome and preferentially with the hyperphosphorylated form of RNA polymerase II (PubMed:12732143, PubMed:22118460, PubMed:32355176, PubMed:34526721, PubMed:38316879). Interacts with ERCC6/CSB (via CIM motif); promoting recruitment to lesion-stalled RNA polymerase II (Pol II) (PubMed:16751180, PubMed:32355176, PubMed:7664335). Interacts with KIAA1530/UVSSA (PubMed:22466612). Interacts with a subunit of RNA polymerase II TFIIH (PubMed:7664335)

Research Field

Synonyms

CKN1; CSA; ERCC8

Documentation

User Guide for Antibodies (1077 KB)