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Interferon alpha/beta Receptor 1 Antibody (YA3112)

(Synonyms: IFNAR1; IFNAR; Interferon alpha/beta receptor 1; IFN-R-1; IFN-alpha/beta receptor 1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; CRF2-1; Type I interferon receptor 1)
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Based on 1 Customer Validation

Interferon alpha/beta Receptor 1 Antibody (YA3112) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Interferon alpha/beta Receptor 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Interferon alpha/beta Receptor 1 Antibody (YA3112)
  • Interferon alpha/beta Receptor 1 Antibody (YA3112) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

Interferon alpha/beta Receptor 1 Antibody (YA3112) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Interferon alpha/beta Receptor 1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 110-130 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 64 kDa
Species Reactivity Database

Entrez Gene: 3454 Human

SwissProt: P17181 Human

Immunogen

A synthetic peptide of human Interferon alpha/beta receptor 1

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105480

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Interferon alpha/beta Receptor 1 Antibody (YA3112)
    Western blot analysis was performed on extracts from K562 (lane 1, 20 μg), Hela (lane 2, 20 μg), 293 (lane 3, 20 μg), and 293T (lane 4, 20 μg) using Interferon alpha/beta Receptor 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    Interferon alpha/beta Receptor 1 is a Together with IFNAR2, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa). Type I interferon binding activates the JAK-STAT signaling cascade, resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response. Mechanistically, type I interferon-binding brings the IFNAR1 and IFNAR2 subunits into close proximity with one another, driving their associated Janus kinases (JAKs) (TYK2 bound to IFNAR1 and JAK1 bound to IFNAR2) to cross-phosphorylate one another. The activated kinases phosphorylate specific tyrosine residues on the intracellular domains of IFNAR1 and IFNAR2, forming docking sites for the STAT transcription factors. STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes. Can also act independently of IFNAR2: form an active IFNB1 receptor by itself and activate a signaling cascade that does not involve activation of the JAK-STAT pathway[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Late endosome; Lysosome

  • Expression


    Tissue_specificity:Interferon receptors are present in all tissues, even on the surface of most interferon-resistant cells. Isomers 1, 2, and 3 are expressed in the interferon-α-sensitive myeloma cell line U266B1. Isomers 2 and 3 are expressed in the interferon-α-resistant myeloma cell line U266R. Isomer 1 is not expressed in the interferon-α-resistant myeloma cell line U266R.

  • Isoforms & Post-Translational Modification

    P17181 has 4 isomers: P17181-1: 63525 Da (predicted); P17181-2: 49547 Da (predicted); P17181-3: 56718 Da (predicted); P17181-4: 56046 Da (predicted).
    Ubiquitinated, leading to its internalization and degradation (PubMed:14532120, PubMed:15337770). Polyubiquitinated via 'Lys-48'-linked and 'Lys-63'-linked ubiquitin chains, leading to receptor internalization and lysosomal degradation (PubMed:18056411). The 'Lys-63'-linked ubiquitin chains are cleaved off by the BRISC complex (PubMed:24075985);Phosphorylated on tyrosine residues in response to interferon-binding: phosphorylation by TYK2 tyrosine kinase creates docking sites for STAT proteins (PubMed:10049744, PubMed:7526154). Phosphorylated on serine residues in response to interferon binding; this promotes interaction with FBXW11 and ubiquitination (PubMed:14532120, PubMed:15337770, PubMed:24075985);Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2

  • Subunit

    Heterodimer with IFNAR2; forming the receptor for type I interferon (PubMed:10049744, PubMed:21854986, PubMed:7665574). Interacts with TYK2 (PubMed:15337770, PubMed:24704786, PubMed:7526154). Interacts with STAT1 and STAT2; the interaction requires its phosphorylation at Tyr-466 (PubMed:9121453). Interacts (serine-phosphorylated form) with FBXW11, the substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex (PubMed:14532120, PubMed:15337770, PubMed:18056411). Interacts with SHMT2; this promotes interaction with ABRAXAS2 and the BRISC complex (PubMed:24075985). Interacts with TRIM10; this interaction prevents association between IFNAR1 and TYK2 (PubMed:33811647)

  • SwissProt ID

    P17181

  • Gene ID
    3454 [NCBI]
  • Synonyms

    IFNAR1; IFNAR; Interferon alpha/beta receptor 1; IFN-R-1; IFN-alpha/beta receptor 1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; CRF2-1; Type I interferon receptor 1

  • Research Field

    Immunology

Interferon alpha/beta Receptor 1 Antibody (YA3112) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from K562 (lane 1, 20 μg), Hela (lane 2, 20 μg), 293 (lane 3, 20 μg), and 293T (lane 4, 20 μg) using Interferon alpha/beta Receptor 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Interferon alpha/beta Receptor 1 Antibody (YA3112) Product Image
    1/16
  • Experimental Validation Results for Interferon alpha/beta Receptor 1 Antibody (YA3112)
  • Interferon alpha/beta Receptor 1 Antibody (YA3112) Product Image
  • Experimental Validation Results for Interferon alpha/beta Receptor 1 Antibody (YA3112)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from K562 (lane 1, 20 μg), Hela (lane 2, 20 μg), 293 (lane 3, 20 μg), and 293T (lane 4, 20 μg) using Interferon alpha/beta Receptor 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Interferon alpha/beta Receptor 1 Antibody (YA3112)
  • Interferon alpha/beta Receptor 1 Antibody (YA3112) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from K562 (lane 1, 20 μg), Hela (lane 2, 20 μg), 293 (lane 3, 20 μg), and 293T (lane 4, 20 μg) using Interferon alpha/beta Receptor 1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Interferon alpha/beta Receptor 1 Antibody (YA3112) Product Image WB