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PLK1 Antibody (YA4084)

(Synonyms: PLK; STPK13)
Customer Review

Based on 1 Customer Validation

PLK1 Antibody (YA4084) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PLK1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PLK1 Antibody (YA4084)
  • PLK1 Antibody (YA4084) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:100-1:500 1:200-1:400 1:10000

Product Details

Description

PLK1 Antibody (YA4084) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PLK1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 68 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 68 kDa
Immunogen

Purified recombinant fragment of human PLK1 (AA: 331-508) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718899

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PLK1 Antibody (YA4084)
    Western blot analysis of extracts from K562(lane 2(20μg), A549(lane 3(20μg), 3T3(lane 4(20μg), A431(lane 5(20μg) using PLK1 Antibody (HY-P84387). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PLK1 is a serine/threonine Polo-like kinase that orchestrates cell division through mitotic entry, spindle-pole functions, and cytokinesis[1]. Mechanistically, mammalian PLK1 controls centrosome maturation, spindle assembly, and microtubule attachment to kinetochores in early mitosis[2]. This mitotic role makes PLK1 relevant to cancer biology, because dysfunction may promote cancerous transformation and drive progression, and overexpression occurs in diverse human cancers with poor prognosis[3]. Compared with related isoforms, PLK1-PLK4 participate in cell-cycle processes, but reported functional emphasis differs: PLK2 and PLK4 regulate centriole duplication, PLK3 regulates DNA replication, and PLK1 regulates centrosome separation and maturation[4]. For experimental applications, PLK1 inhibition provides a direct way to perturb mitosis, because BI 2536 inhibits mammalian PLK1 at low nanomolar concentrations, induces mitotic arrest and apoptosis in human cancer cell lines, and inhibits human tumor xenograft growth in nude mice[5].

  • Subcellular Localization

    Nucleus; Chromosome, centromere, kinetochore; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle; Midbody

  • Expression


    Tissue_specificity:placenta and colon

    Induction:By growth-stimulating agents

  • Subunit

    Interacts with CEP170 (PubMed:15616186). Interacts with EVI5 (PubMed:16439210). Interacts with FAM29A (PubMed:19029337). Interacts with SLX4/BTBD12 (PubMed:19596235). Interacts with TTDN1 (PubMed:17310276). Interacts (via POLO-box domain) with the phosphorylated form of BUB1, CDC25C and CENPU (PubMed:14532005, PubMed:16760428, PubMed:17307877, PubMed:19597481). Interacts with KIF2A (PubMed:19351716). Interacts with CYLD (PubMed:17495026). Part of an astrin (SPAG5)-kinastrin (SKAP) complex containing KNSTRN, SPAG5, PLK1, DYNLL1 and SGO2 (PubMed:21402792). Interacts with BIRC6/bruce (PubMed:18329369). Interacts with CDK1-phosphorylated FRY; this interaction occurs in mitotic cells, but not in interphase cells (PubMed:22753416). FRY interaction facilitates AURKA-mediated PLK1 phosphorylation (PubMed:22753416). Interacts with CDK1-phosphorylated DCTN6 during mitotic prometaphase; the interaction facilitates recruitment to kinetochores (PubMed:23455152). Interacts with CEP68; the interaction phosphorylates CEP68 (PubMed:25503564). Interacts (via POLO-box domain) with DCTN1 (PubMed:20679239). Interacts with CEP20 in later G1, S, G2 and M phases of the cell cycle; this interaction recruits PLK1 to centrosomes, a step required for S phase progression (PubMed:24018379). Interacts with KLHL22 (PubMed:23455478, PubMed:24067371). Interacts (via POLO box domains) with NEDD9/HEF1 (via C-terminus) (PubMed:29191835). Interacts with FIRRM (via N-terminus region); required for maintaining, but not activating, PLK1 kinase activity (PubMed:34260926). Interacts with FZR1 (PubMed:18662541). Interacts with SKA3; the interaction promotes the stability of PLK1; the interaction promotes the stability of PLK1 (PubMed:32799774). Interacts with the MTMR3:MTMR4 heterooligomer; brings CEP55 and PLK1 together during early mitosis, regulating the phosphorylation of CEP55 by PLK1 and its recruitment to the midbody where it can mediate cell abscission (PubMed:25659891)

  • SwissProt ID

    P53350

  • Gene ID
    5347 [NCBI]
  • Synonyms

    PLK; STPK13

References

[1]. Barr FA, et al. Polo-like kinases and the orchestration of cell division. Nat Rev Mol Cell Biol. 2004 Jun;5(6):429-40. [Content Brief]

[2]. Santamaria A, et al. The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics. 2011 Jan;10(1):M110.004457. [Content Brief]

[3]. Liu Z, et al. PLK1, A Potential Target for Cancer Therapy. Transl Oncol. 2017 Feb;10(1):22-32. [Content Brief]

[4]. de Cárcer G, et al. From Plk1 to Plk5: functional evolution of polo-like kinases. Cell Cycle. 2011 Jul 15;10(14):2255-62. [Content Brief]

[5]. Steegmaier M, et al. BI 2536, a potent and selective inhibitor of polo-like kinase 1, inhibits tumor growth in vivo. Curr Biol. 2007 Feb 20;17(4):316-22. [Content Brief]

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PLK1 Antibody (YA4084) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20μg), A549(lane 3(20μg), 3T3(lane 4(20μg), A431(lane 5(20μg) using PLK1 Antibody (HY-P84387). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • PLK1 Antibody (YA4084) Product Image
    1/16
  • Experimental Validation Results for PLK1 Antibody (YA4084)
  • PLK1 Antibody (YA4084) Product Image
  • Experimental Validation Results for PLK1 Antibody (YA4084)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20μg), A549(lane 3(20μg), 3T3(lane 4(20μg), A431(lane 5(20μg) using PLK1 Antibody (HY-P84387). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PLK1 Antibody (YA4084)
  • PLK1 Antibody (YA4084) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20μg), A549(lane 3(20μg), 3T3(lane 4(20μg), A431(lane 5(20μg) using PLK1 Antibody (HY-P84387). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • PLK1 Antibody (YA4084) Product Image WB