HNF 4 alpha Antibody (YA1190)
(Synonyms: TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; TCF-14; HNF4alpha)
Based on 1 Customer Validation
HNF 4 alpha Antibody (YA1190) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HNF 4 alpha.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IP, ChIP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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ChIP
ChIP: Chromatin Immunoprecipitation
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:20 | 1:20 | 1:50-1:100 |
Product Details
HNF 4 alpha Antibody (YA1190) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HNF 4 alpha.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 53 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 53 kDa
Entrez Gene: 3172 Human ; 15378 Mouse ; 25735 Rat
SwissProt: P41235 Human ; P49698 Mouse ; P22449 Rat
OMIM: 125850 Human
Recombinant protein of mouse HNF-4-alpha
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
HNF 4 alpha transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitatating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38. Represses the CLOCK-BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells[1][2].
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:Expressed in the liver, pancreas and colon in a circadian manner -
Isoforms & Post-Translational Modification
P49698 has 2 isomers: P49698-1: 52684 Da (predicted); P49698-2: 51518 Da (predicted).
Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA (By similarity);Acetylation at Lys-458 lowers transcriptional activation by about two-fold -
Subunit
Homodimerization is required for HNF4-alpha to bind to its recognition site (By similarity). Interacts with CLOCK, BMAL1 and PER1 (By similarity). Interacts with PER2 (PubMed:20159955). Interacts with CRY1 and CRY2 (PubMed:28751364). Interacts with NR0B2/SHP; the resulting heterodimer is transcriptionally inactive (By similarity). Interacts with DDX3X; this interaction disrupts the interaction between HNF4 and NR0B2 that forms inactive heterodimers and enhances the formation of active HNF4 homodimers (By similarity)
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SwissProt ID
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Synonyms
TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; TCF-14; HNF4alpha
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Research Field
Cardiovascular
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