HNF 4 alpha Antibody (YA1190)

製品番号: HY-P81445: データシート SDS COA User Guide for Antibodies
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Technical Support

HNF 4 alpha Antibody (YA1190) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HNF 4 alpha.

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容量	価格（税別）	在庫状況	数量
20 μL	$146	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明

製品説明

HNF 4 alpha Antibody (YA1190) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HNF 4 alpha.

Host

Rabbit

Clonality

Recombinant,Monoclonal

分子量

Predicted band size: 53 kDa;

Observed band size: 53 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P49698

Gene ID

3172 [NCBI]

Immunogen

Recombinant protein of mouse HNF-4-alpha

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20
ChIP ChIP: Chromatin Immunoprecipitation	1:20
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Solution

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitatating the recruitment of RNA pol II to the promoters of target genes (By similarity). Activates the transcription of CYP2C38 (PubMed:30555544). Represses the CLOCK-BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells (PubMed:30530698)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:Expressed in the liver, pancreas and colon in a circadian manner

Isoforms & Post-Translational Modification：P49698 has 2 isomers: P49698-1: 52684 Da (predicted); P49698-2: 51518 Da (predicted).
Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA (By similarity);Acetylation at Lys-458 lowers transcriptional activation by about two-fold

Subunit：Homodimerization is required for HNF4-alpha to bind to its recognition site (By similarity). Interacts with CLOCK, BMAL1 and PER1 (By similarity). Interacts with PER2 (PubMed:20159955). Interacts with CRY1 and CRY2 (PubMed:28751364). Interacts with NR0B2/SHP; the resulting heterodimer is transcriptionally inactive (By similarity). Interacts with DDX3X; this interaction disrupts the interaction between HNF4 and NR0B2 that forms inactive heterodimers and enhances the formation of active HNF4 homodimers (By similarity)

Database

Entrez Gene: 3172 Human ; 15378 Mouse ; 25735 Rat

SwissProt: P41235 Human ; P49698 Mouse ; P22449 Rat

OMIM: 125850 Human

Research Field

Cardiovascular

Synonyms

TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; TCF-14; HNF4alpha

ドキュメンテーション

Select Batch:

データシート (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)