Phospho-Histone H2A.X (Ser139) Antibody (YA191)(PBS only)

Cat. No.: HY-P80821A: Fiche technique User Guide for Antibodies
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Phospho-Histone H2A.X (Ser139) Antibody (YA191) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Histone H2A.X (Ser139).

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Size		Stock
50 μL		Obtenir un devis
100 μL		Obtenir un devis

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description

Description

Phospho-Histone H2A.X (Ser139) Antibody (YA191) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Histone H2A.X (Ser139).

Host

Rabbit

Masse moléculaire

Predicted band size: 15 kDa;

Observed band size: 15 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P16104

Gene ID

3014 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser139 of Human gamma H2A.X.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Pureté	affinity purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation

Subcellular Localization：Nucleus; Chromosome

Subunit：The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA (Probable). Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140 (PubMed:12419185, PubMed:12607005, PubMed:15201865). These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11, RAD50, and NBN (PubMed:12419185, PubMed:12607005, PubMed:15201865). Interaction with the MRN complex is mediated at least in part by NBN (PubMed:12419185). Also interacts with DHX9/NDHII when phosphorylated on Ser-140 and MCPH1 when phosphorylated at Ser-140 or Tyr-143 (PubMed:15613478). Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation (PubMed:16820410). Interacts with WRAP53/TCAB1 (PubMed:26734725, PubMed:27715493). Interacts with HDGFL2 (PubMed:26721387). Interacts with DNA damage up-regulated protein DDUP (PubMed:35849344). Forms a complex with DDUP and RAD18 following DDUP phosphorylation (PubMed:35849344)

Research Field

Epigenetics and Nuclear Signaling

Synonyms

H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; gamma H2A.X

Documentation

User Guide for Antibodies (1077 KB)