Collagen I alpha 2 Antibody (YA3695)(PBS only)

Cat. No.: HY-P83998A: Data Sheet User Guide for Antibodies
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Collagen I alpha 2 Antibody (YA3695) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Collagen I alpha 2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Collagen I alpha 2 Antibody (YA3695) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Collagen I alpha 2.

Host

Mouse

Molecular Weight

Predicted band size: 129 kDa;

Observed band size: 150 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey

SwissProt ID

P08123

Gene ID

1278 [NCBI]

Immunogen

Purified recombinant fragment of human COL1A2 (AA: 23-79) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Type I collagen is a member of group I collagen (fibrillar forming collagen)

Subcellular Localization：Secreted, extracellular space, extracellular matrix

Expression：
Tissue_specificity:The fibers that make up tendons, ligaments, and bones. In bones, these fibers are mineralized by hydroxyapatite calcium.

Subunit：Trimers of one alpha 2(I) and two alpha 1(I) chains (Probable). Interacts (via C-terminus) with TMEM131 (via PapD-L domain); the interaction is direct and is involved in assembly and TRAPPIII ER-to-Golgi transport complex-dependent secretion of collagen (PubMed:32095531)

Synonyms

OI4; EDSCV; EDSARTH2

Documentation

User Guide for Antibodies (1077 KB)