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CD11c Antibody (YA3389)

(Synonyms: p150/95 integrin alpha chain; Leu M5; SLEB6; ITGAX; CD11c; p150; Myeloid membrane antigen alpha subunit; Leukocyte surface antigen p150 95 alpha subunit; Integrin alpha-X; Integrin alpha X)
Customer Review

Based on 1 Customer Validation

CD11c Antibody (YA3389) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD11c.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • CD11c Antibody (YA3389) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:200 1:50

Product Details

Description

CD11c Antibody (YA3389) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD11c.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 140 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 128 kDa
Species Reactivity Database

Entrez Gene: 3687 Human

SwissProt: P20702 Human

Immunogen

A synthesized peptide derived from human CD11c aa1100-1163.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD11c Antibody (YA3389)
    Western blot analysis was performed on extracts from THP-1 (lane 1, 15 μg) using CD11c Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CD11c Antibody (YA3389)
    Immunohistochemical analysis of paraffin-embedded human ‌Kimura's disease tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD11c Antibody (YA3389)
    Immunohistochemical analysis of paraffin-embedded human ‌Follicular Lymphoma tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD11c Antibody (YA3389)
    Immunohistochemical analysis of paraffin-embedded human ‌Liver cyst tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD11c Antibody (YA3389)
    Immunohistochemical analysis of paraffin-embedded human ‌Hepatocellular Carcinoma tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD11c Antibody (YA3389)
    Immunohistochemical analysis of paraffin-embedded human ‌Glioma‌ tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD11c Antibody (YA3389)
    Immunohistochemical analysis of paraffin-embedded human ‌Squamous Cell Carcinoma tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    CD11c is an integrin α-chain protein, belonging to the integrin α subunit family. CD11c usually forms a heterodimer αXβ2 integrin with CD18 (Integrin β2, ITGB2). CD11c is one of the most commonly used dendritic cell (DC) markers in immunological research, but it is not actually expressed specifically by DCs; it is expressed in various myeloid immune cells.

  • Subcellular Localization

    Membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is mainly expressed in monocytes and granulocytes.

  • Subunit

    Heterodimer of an alpha and a beta subunit. Alpha-X associates with beta-2

  • SwissProt ID

    P20702

  • Gene ID
    3687 [NCBI]
  • Synonyms

    p150/95 integrin alpha chain; Leu M5; SLEB6; ITGAX; CD11c; p150; Myeloid membrane antigen alpha subunit; Leukocyte surface antigen p150 95 alpha subunit; Integrin alpha-X; Integrin alpha X

CD11c Antibody (YA3389) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from THP-1 (lane 1, 15 μg) using CD11c Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Kimura's disease tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Follicular Lymphoma tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Liver cyst tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Hepatocellular Carcinoma tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Glioma‌ tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Squamous Cell Carcinoma tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD11c Antibody (YA3389) Product Image
    1/16
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • CD11c Antibody (YA3389) Product Image
  • Experimental Validation Results for CD11c Antibody (YA3389)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from THP-1 (lane 1, 15 μg) using CD11c Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CD11c Antibody (YA3389)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Kimura's disease tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD11c Antibody (YA3389)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Follicular Lymphoma tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD11c Antibody (YA3389)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Liver cyst tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD11c Antibody (YA3389)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Hepatocellular Carcinoma tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD11c Antibody (YA3389)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Glioma‌ tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD11c Antibody (YA3389)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Squamous Cell Carcinoma tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • Experimental Validation Results for CD11c Antibody (YA3389)
  • CD11c Antibody (YA3389) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from THP-1 (lane 1, 15 μg) using CD11c Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • CD11c Antibody (YA3389) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Kimura's disease tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD11c Antibody (YA3389) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Follicular Lymphoma tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD11c Antibody (YA3389) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Liver cyst tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD11c Antibody (YA3389) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Hepatocellular Carcinoma tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD11c Antibody (YA3389) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Glioma‌ tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD11c Antibody (YA3389) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Squamous Cell Carcinoma tissue using CD11c antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P83653, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • CD11c Antibody (YA3389) Product Image WB
  • CD11c Antibody (YA3389) Product Image IHC-P
  • CD11c Antibody (YA3389) Product Image IHC-P
  • CD11c Antibody (YA3389) Product Image IHC-P
  • CD11c Antibody (YA3389) Product Image IHC-P
  • CD11c Antibody (YA3389) Product Image IHC-P
  • CD11c Antibody (YA3389) Product Image IHC-P