NF2 Antibody (YA7467)
Based on 1 Customer Validation
NF2 Antibody (YA7467) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to NF2.
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Host:
Rabbit
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Isotype:
IgG,Kappa
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Application:
WB, ICC/IF, IP, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 | 1:50-1:200 |
Product Details
NF2 Antibody (YA7467) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to NF2.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 70 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 70 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG,Kappa
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NF2 is a Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex
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Subcellular Localization
Cell projection, filopodium membrane,Cell projection, ruffle membrane,Nucleus,Cytoplasm, perinuclear region,Cytoplasmic granule,Cytoplasm, perinuclear region,Cytoplasmic granule,Nucleus,Cell projection, filopodium membrane,Cell projection, ruffle membrane,Cytoplasm, perinuclear region,Cytoplasmic granule,Cytoplasm, cytoskeleton
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Expression
Tissue_Specificity: Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels -
Isoforms & Post-Translational Modification
P35240 has 10 isomers: P35240-1: 69690 Da (predicted); P35240-2: 72514 Da (predicted); P35240-3: 69090 Da (predicted); P35240-4: 59096 Da (predicted); P35240-5: 64189 Da (predicted); P35240-6: 63996 Da (predicted); P35240-7: 30454 Da (predicted); P35240-8: 65350 Da (predicted); P35240-9: 19215 Da (predicted); P35240-10: 24515 Da (predicted).
Phosphorylation of Ser-518 inhibits nuclear localization by disrupting the intramolecular association of the FERM domain with the C-terminal tail (PubMed:20178741)丨Ubiquitinated by the CUL4A-RBX1-DDB1-DCAF1/VprBP E3 ubiquitin-protein ligase complex for ubiquitination and subsequent proteasome-dependent degradation -
Subunit
Interacts with NHERF1, HGS and AGAP2
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SwissProt ID
Documentation
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