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  4. Monoclonal Antibodies
  5. CBP Tag Antibody (YA1314)

CBP Tag Antibody (YA1314)

Cat. No.: HY-P81569
COA
SDS
User Guide for Antibodies Technical Support

CBP Tag Antibody (YA1314) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CBP Tag.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • Documentation

Description

CBP Tag Antibody (YA1314) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CBP Tag.
Host

Mouse
Clonality

Monoclonal
Molecular Weight
Predicted band size: 55 kDa;
Observed band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Species independent
SwissProt ID

Gene ID
Immunogen

Recombinant Protein of CBP Tag
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:10000
Purity Affinity purified Conjugation Non-conjugated
Modification Unmodified Isotype IgG1
Appearance

Liquid
Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on extracts from Recombinant CBP-Tag protein-10ng (lane 1, 10 ng), Recombinant CBP-Tag protein-5ng (lane 2, 5 ng), Recombinant CBP-Tag protein-2ng (lane 3, 2 ng), and Recombinant CBP-Tag protein-1ng (lane 4, 1 ng) using CBP Tag Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Mouse IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background
Function：CBP affinity tag is a useful tag in protein study which can be added to the N or C terminus of proteins of interest through DNA recombinant technology. The tag is derived from muscle myosin light-chain kinase. The tag comprises 26 amino acid residues with the molecular weight of 4 kDa and sequence of KRRWKKNFIAVSAANRFKKISSSGAL. CBP tag has the relatively high affinity for calmodulin (CaM).
RRID
Research Field

Epigenetics
Synonyms
calmodulin binding peptide,KRRWKKNFIAVSAANRFKKISSSGAL
Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

CBP Tag Antibody (YA1314) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CBP Tag Antibody (YA1314)
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