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Angiopoietin 1 Antibody

Angiopoietin 1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Angiopoietin 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Angiopoietin 1 Antibody
  • Angiopoietin 1 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Angiopoietin 1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Angiopoietin 1.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Species Reactivity Database
Immunogen

The antiserum was produced against synthesized peptide derived from the N-terminal region of human ANGPT1.

Sensitivity

Endogenous

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Angiopoietin 1 Antibody
    Western blot analysis of extracts from Jurkat(lane 2(20ug) , C6(lane 3(20ug) and Hela(lane 4(20ug)using Angiopoietin 1 Antibody (HY-P81847) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Angiopoietin 1 binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme

  • Subcellular Localization

    Secreted

  • Isoforms & Post-Translational Modification

    Q15389 has 2 isomers: Q15389-1: 57513 Da (predicted); Q15389-2: 57456 Da (predicted).
    Glycosylated

  • Subunit

    Homooligomer (PubMed:28601681). Interacts with TEK/TIE2 (PubMed:28601681, PubMed:30689269). Interacts with SVEP1/polydom (By similarity). Interacts with THBD; this interaction significantly inhibits the generation of activated PC and TAFIa/CPB2 by the thrombin/thrombomodulin complex (PubMed:29323190)

  • SwissProt ID

    Q15389

  • Gene ID
    284 [NCBI]
  • Research Field

    Cancer

Angiopoietin 1 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) , C6(lane 3(20ug) and Hela(lane 4(20ug)using Angiopoietin 1 Antibody (HY-P81847) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Angiopoietin 1 Antibody Product Image
    1/16
  • Experimental Validation Results for Angiopoietin 1 Antibody
  • Angiopoietin 1 Antibody Product Image
  • Experimental Validation Results for Angiopoietin 1 Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) , C6(lane 3(20ug) and Hela(lane 4(20ug)using Angiopoietin 1 Antibody (HY-P81847) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Angiopoietin 1 Antibody
  • Angiopoietin 1 Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2(20ug) , C6(lane 3(20ug) and Hela(lane 4(20ug)using Angiopoietin 1 Antibody (HY-P81847) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Angiopoietin 1 Antibody Product Image WB