Cofilin Antibody (YA1311)

Cat. No.: HY-P81566: Data Sheet COA
SDS
User Guide for Antibodies
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Technical Support

Cofilin Antibody (YA1311) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cofilin.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Cofilin Antibody (YA1311) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation
References

Description

Cofilin Antibody (YA1311) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cofilin.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 19 kDa;

Observed band size:19 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey

SwissProt ID

P23528

Gene ID

1072 [NCBI]

Immunogen

Synthetic peptide within human Cofilin aa 117-166 / 166.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
FC FC: Flow Cytometry	1:200-1:1000

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 1(20μg)) 、MCF7 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using Cofilin Antibody (HY-P81566) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity)

Subcellular Localization：Nucleus matrix; Cytoplasm, cytoskeleton; Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, lamellipodium; Cell projection, growth cone; Cell projection, axon

Expression：
Tissue_specificity:Widely distributed in various tissues

Induction:Up-regulated in response to enterovirus 71 (EV71) infection (at protein level)

Positive sample: Hela cell lysate, HEK-293 cell lysate, MCF-7 cell lysate, MDA-MB-468 cell lysate, K-562 cell lysate, SH-SY5Y cell lysate, HUVEC cell lysate, Jurkat cell lysate,

Subunit：Can bind G- and F-actin in a 1:1 ratio of cofilin to actin (PubMed:11812157). It is a major component of intranuclear and cytoplasmic actin rods (By similarity). Interacts with the subcortical maternal complex (SCMC) via interaction with TLE6 isoform 1 and NLRP5 (By similarity). Interacts with C9orf72 (By similarity)

RRID

AB_3103678

Database

Entrez Gene: 1072 Human ; 12631 Mouse ; 29271 Rat

SwissProt: P23528 Human ; P18760 Mouse ; P45592 Rat

OMIM: 601442 Human

Research Field

Tumor biology

Synonyms

18 kDa phosphoprotein antibody; CFL 1 antibody; CFL antibody; CFL1 antibody; COF1_HUMAN antibody; Cofilin 1 antibody; Cofilin 1 non muscle antibody; Cofilin antibody; Cofilin non muscle isoform antibody; Cofilin-1 antibody; epididymis secretory protein Li 15 antibody; HEL-S-15 antibody; non-muscle isoform antibody; p18 antibody

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/sk-ov-3-cell.html [Content Brief]

Cofilin Antibody (YA1311) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cofilin Antibody (YA1311)

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Background

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Cofilin Antibody (YA1311) Related Classifications

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