Transmembrane Protein 85 Antibody (YA2225)(PBS only)

Cat. No.: HY-P82480A: Data Sheet User Guide for Antibodies
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Transmembrane Protein 85 Antibody (YA2225) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transmembrane Protein 85.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

Transmembrane Protein 85 Antibody (YA2225) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transmembrane Protein 85.

Host

Rabbit

Molecular Weight

Predicted band size: 20 kDa;

Observed band size: 20 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Hamster

SwissProt ID

Q5J8M3

Gene ID

51234 [NCBI]

Immunogen

A synthetic peptide of human TMEM85 aa42-55.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:29242231, PubMed:29809151, PubMed:30415835, PubMed:32439656, PubMed:32459176). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:29242231, PubMed:29809151, PubMed:30415835). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:29809151, PubMed:30415835). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29242231, PubMed:29809151). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable)

Subcellular Localization：Endoplasmic reticulum membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Isoform 1 is expressed in brain and heart. Isoform 2 is expressed in heart

Isoforms & Post-Translational Modification：Q5J8M3 has 3 isomers: Q5J8M3-1: 20087 Da (predicted); Q5J8M3-2: 16745 Da (predicted); Q5J8M3-3: 15088 Da (predicted).

Subunit：Component of the ER membrane protein complex (EMC)

Research Field

Cell Biology

Synonyms

PIG17; TMEM85

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/a549-cell.html [Content Brief]