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PAX2 Antibody (YA2818)

(Synonyms: PAX2; Paired box protein Pax-2)
Customer Review

Based on 1 Customer Validation

PAX2 Antibody (YA2818) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAX2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Liquid in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL IHC-P FC
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • PAX2 Antibody (YA2818) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:100

Product Details

Description

PAX2 Antibody (YA2818) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAX2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Pax2 aa150-350.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Liquid in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PAX2 Antibody (YA2818)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAX2 Antibody (YA2818)
    Immunohistochemical analysis of paraffin-embedded human seminal vesicle tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAX2 Antibody (YA2818)
    Immunohistochemical analysis of paraffin-embedded human epididymis tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAX2 Antibody (YA2818)
    Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAX2 Antibody (YA2818)
    Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAX2 Antibody (YA2818)
    Flow cytometric analysis of 1X106 K562 cells labeling PAX2 Antibody (HY-P83073, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    PAX2 transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS[1].

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:It is expressed in primitive cells of the kidneys, ureters, eyes, ears, and central nervous system.

  • Isoforms & Post-Translational Modification

    Q02962 has 4 isomers: Q02962-1: 44706 Da (predicted); Q02962-2: 44485 Da (predicted); Q02962-3: 42177 Da (predicted); Q02962-4: 41956 Da (predicted).

  • Subunit

    Interacts with ELGN3; the interaction targets PAX2 for destruction. Interacts with TLE4

  • SwissProt ID

    Q02962

  • Gene ID
    5076 [NCBI]
  • Synonyms

    PAX2; Paired box protein Pax-2

  • Research Field

    Neuroscience

PAX2 Antibody (YA2818) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human seminal vesicle tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human epididymis tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling PAX2 Antibody (HY-P83073, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • PAX2 Antibody (YA2818) Product Image
    1/16
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • PAX2 Antibody (YA2818) Product Image
  • Experimental Validation Results for PAX2 Antibody (YA2818)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAX2 Antibody (YA2818)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human seminal vesicle tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAX2 Antibody (YA2818)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human epididymis tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAX2 Antibody (YA2818)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAX2 Antibody (YA2818)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PAX2 Antibody (YA2818)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling PAX2 Antibody (HY-P83073, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • Experimental Validation Results for PAX2 Antibody (YA2818)
  • PAX2 Antibody (YA2818) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PAX2 Antibody (YA2818) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human seminal vesicle tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PAX2 Antibody (YA2818) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human epididymis tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PAX2 Antibody (YA2818) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PAX2 Antibody (YA2818) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human renal cancer tissue using PAX2 Antibody (HY-P83073, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PAX2 Antibody (YA2818) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 K562 cells labeling PAX2 Antibody (HY-P83073, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • PAX2 Antibody (YA2818) Product Image IHC-P
  • PAX2 Antibody (YA2818) Product Image IHC-P
  • PAX2 Antibody (YA2818) Product Image IHC-P
  • PAX2 Antibody (YA2818) Product Image IHC-P
  • PAX2 Antibody (YA2818) Product Image IHC-P
  • PAX2 Antibody (YA2818) Product Image FC