Phospho-FOXO3A(Ser253) Antibody (YA196)

Cat. No.: HY-P80461: Data Sheet COA
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Phospho-FOXO3A(Ser253) Antibody (YA196) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-FOXO3A(Ser253).

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Phospho-FOXO3A(Ser253) Antibody (YA196) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Phospho-FOXO3A(Ser253) Antibody (YA196) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-FOXO3A(Ser253).

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 71 kDa;

Observed band size: 100 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O43524

Gene ID

2309 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser252 of Human Metabotropic Glutamate Receptor 5.AA range:238-282.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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ALL WB IHC-P

Western blot analysis of extracts from Hela (lane 2(20μg), THP-1 (lane 3(20μg), K562 (lane 4(20μg),using Phospho-FOXO3A(Ser253)Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using Phospho-FOXO3A(Ser253) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using Phospho-FOXO3A(Ser253) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using Phospho-FOXO3A(Ser253) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy (PubMed:10102273, PubMed:16751106, PubMed:21329882, PubMed:30513302). Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins (By similarity). Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PubMed:10102273, PubMed:16751106). Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PubMed:21329882). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed:23283301). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Also acts as a key regulator of regulatory T-cells (Treg) differentiation by activating expression of FOXP3 (PubMed:30513302)

Subcellular Localization：Cytoplasm, cytosol; Nucleus; Mitochondrion matrix; Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side

Expression：
Tissue_specificity:general expression

Subunit：Upon metabolic stress, forms a complex composed of FOXO3, SIRT3 and mitochondrial RNA polymerase POLRMT; the complex is recruited to mtDNA in a SIRT3-dependent manner (PubMed:23283301). Also forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT (PubMed:29445193). Interacts with SIRT2; the interaction occurs independently of SIRT2 deacetylase activity (By similarity). Interacts with YWHAB/14-3-3-beta and YWHAZ/14-3-3-zeta, which are required for cytosolic sequestration (PubMed:16751106). Upon oxidative stress, interacts with STK4/MST1, which disrupts interaction with YWHAB/14-3-3-beta and leads to nuclear translocation (PubMed:16751106). Interacts with PIM1 (PubMed:18593906). Interacts with DDIT3/CHOP (PubMed:22761832). Interacts (deacetylated form) with SKP2 (PubMed:21841822). Interacts with CHUK and IKBKB (PubMed:15084260, PubMed:22313691). Interacts with CAMK2A, CAMK2B and calcineurin A (By similarity). Interacts with NUPR1; this interaction represses FOXO3 transactivation (PubMed:20181828)

RRID

AB_3102535

Database

Entrez Gene: 2309 Human ; 56484 Mouse ; 294515 Rat

SwissProt: O43524 Human ; Q9WVH4 Mouse ; D3ZBQ1 Rat

OMIM: 602681 Human

Research Field

Epigenetics and Nuclear Signaling

Documentation

Select Batch:

Data Sheet (235 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-FOXO3A(Ser253) Antibody (YA196) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-FOXO3A(Ser253) Antibody (YA196)

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Background

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Phospho-FOXO3A(Ser253) Antibody (YA196) Related Classifications

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