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EAAT2 Antibody (YA2482)

(Synonyms: Excitatory amino acid transporter 2; Glutamate/aspartate transporter II; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2)
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Based on 1 Customer Validation

EAAT2 Antibody (YA2482) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EAAT2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for EAAT2 Antibody (YA2482)
  • EAAT2 Antibody (YA2482) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50

Product Details

Description

EAAT2 Antibody (YA2482) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EAAT2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 62 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human EAAT2 aa150-250.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104835

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EAAT2 Antibody (YA2482)
    Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), K562 (lane 2, 20 μg),SH-SY5Y (lane 3, 20 μg), U87MG (lane 4, 20 μg) and PC-12 (lane 5, 20 μg) using EAAT2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    The excitatory amino acid transporter 2 (EAAT2) serves as the primary glutamate transporter in the human central nervous system, regulating extracellular glutamate concentrations and maintaining excitatory-inhibitory homeostasis[1]. Mechanistically, EAAT2 clears synaptic glutamate by uptake into astrocytes, facilitating recycling through glutamine synthesis[1]. Compared with related isoforms, such as EAAT1, EAAT2 exhibits higher expression in cortical and hippocampal astrocytes and displays greater transport capacity for excitatory neurotransmission[1]. Dysregulation of EAAT2 expression or localization contributes to neurodegenerative processes, with experimental models of Alzheimer's disease demonstrating altered EAAT2 distribution and correlation with cognitive deficits[1]. EAAT2-specific agonists or modulators have been employed in preclinical studies to restore glutamate homeostasis, providing neuroprotective effects and highlighting potential translational applications[1]. Therefore, EAAT2 functions as a key molecular target for studying synaptic excitatory control, neurodegeneration, and pharmacological modulation of glutamatergic signaling in disease-relevant contexts[1].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Isoforms & Post-Translational Modification

    P43004 has 3 isomers: P43004-1: 62104 Da (predicted); P43004-2: 61228 Da (predicted); P43004-3: 61008 Da (predicted).
    Glycosylated;Palmitoylation at Cys-38 is not required for correct subcellular localization, but is important for glutamate uptake activity

  • Subunit

    Homotrimer (PubMed:15265858, PubMed:15483603, PubMed:26483543). Isoform 3 can oligomerize with isoform 1 (PubMed:20688910).

  • SwissProt ID

    P43004

  • Gene ID
    6506 [NCBI]
  • Synonyms

    Excitatory amino acid transporter 2; Glutamate/aspartate transporter II; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2

  • Research Field

    Neuroscience

[1]. Wood OWG, et al. EAAT2 as a therapeutic research target in Alzheimer's disease: A systematic review. Front Neurosci. 2022 Aug 10;16:952096. [Content Brief]

EAAT2 Antibody (YA2482) Related Classifications

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Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), K562 (lane 2, 20 μg),SH-SY5Y (lane 3, 20 μg), U87MG (lane 4, 20 μg) and PC-12 (lane 5, 20 μg) using EAAT2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • EAAT2 Antibody (YA2482) Product Image
    1/16
  • Experimental Validation Results for EAAT2 Antibody (YA2482)
  • EAAT2 Antibody (YA2482) Product Image
  • Experimental Validation Results for EAAT2 Antibody (YA2482)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), K562 (lane 2, 20 μg),SH-SY5Y (lane 3, 20 μg), U87MG (lane 4, 20 μg) and PC-12 (lane 5, 20 μg) using EAAT2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for EAAT2 Antibody (YA2482)
  • EAAT2 Antibody (YA2482) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), K562 (lane 2, 20 μg),SH-SY5Y (lane 3, 20 μg), U87MG (lane 4, 20 μg) and PC-12 (lane 5, 20 μg) using EAAT2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • EAAT2 Antibody (YA2482) Product Image WB