IL-11 Receptor alpha Antibody (YA3092)

(Synonyms: IL11RA; Interleukin-11 receptor subunit alpha; IL-11 receptor subunit alpha; IL-11R subunit alpha; IL-11R-alpha; IL-11RA)
Customer Review

Based on 1 Customer Validation

IL-11 Receptor alpha Antibody (YA3092) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-11 Receptor alpha.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

IL-11 Receptor alpha Antibody (YA3092) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-11 Receptor alpha.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 45 kDa
Species Reactivity Database

Entrez Gene: 3590 Human

SwissProt: Q14626 Human

Immunogen

A synthetic peptide of human IL11RA

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105460

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IL-11 Receptor alpha Antibody (YA3092)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using IL-11 Receptor alpha Antibody (HY-P83347, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-11 Receptor alpha Antibody (YA3092)
    Immunohistochemical analysis of paraffin-embedded human epididymis tissue using IL-11 Receptor alpha Antibody (HY-P83347, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-11 Receptor alpha Antibody (YA3092)
    Immunohistochemical analysis of paraffin-embedded human prostate tissue using IL-11 Receptor alpha Antibody (HY-P83347, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-11 Receptor alpha Antibody (YA3092)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using IL-11 Receptor alpha Antibody (HY-P83347, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-11 Receptor alpha Antibody (YA3092)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using IL-11 Receptor alpha Antibody (HY-P83347, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-11 Receptor alpha Antibody (YA3092)
    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using IL-11 Receptor alpha Antibody (HY-P83347, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-11 Receptor alpha Antibody (YA3092)
    Immunohistochemical analysis of paraffin-embedded human stomach tissue using IL-11 Receptor alpha Antibody (HY-P83347, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-11 Receptor alpha Antibody (YA3092)
    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using IL-11 Receptor alpha Antibody (HY-P83347, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-11 Receptor alpha Antibody (YA3092)
    Immunohistochemical analysis of paraffin-embedded human liver tissue using IL-11 Receptor alpha Antibody (HY-P83347, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-11 Receptor alpha Antibody (YA3092)
    Immunohistochemical analysis of paraffin-embedded human ovary tissue using IL-11 Receptor alpha Antibody (HY-P83347, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-11 Receptor alpha Antibody (YA3092)
    Immunohistochemical analysis of paraffin-embedded human oral mucosa tissue using IL-11 Receptor alpha Antibody (HY-P83347, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-11 Receptor alpha Antibody (YA3092)
    Immunohistochemical analysis of paraffin-embedded human testis tissue using IL-11 Receptor alpha Antibody (HY-P83347, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    IL-11 Receptor alpha receptor for interleukin-11 (IL11). The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. The IL11/IL11RA/IL6ST complex may be involved in the control of proliferation and/or differentiation of skeletogenic progenitor or other mesenchymal cells (Probable). Essential for the normal development of craniofacial bones and teeth. Restricts suture fusion and tooth number; Soluble form of IL11 receptor (sIL11RA) that acts as an agonist of IL11 activity. The IL11:sIL11RA complex binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL11RA in a process called IL11 trans-signaling; Soluble form of IL11 receptor (sIL11RA) that acts as an agonist of IL11 activity. The IL11:sIL11RA complex binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL11RA in a process called IL11 trans-signaling[1][2].

  • Subcellular Localization

    Membrane; Single-pass type I membrane protein; Secreted; Secreted

  • Expression


    Tissue_specificity:Expressed in a number of cell lines, including the myelogenous leukemia cell line K-562, the megakaryocytic leukemia cell line M-07e, the erythroleukemia cell line TF-1, and the osteosarcoma cell lines, MG-63 and SaOS-2 (PubMed:7670098) . Also expressed in normal and malignant prostate epithelial cell lines. Expression levels are increased in prostate carcinoma

  • Isoforms & Post-Translational Modification

    Q14626 has 2 isomers: Q14626-1: 45222 Da (predicted); Q14626-2: 41842 Da (predicted).
    A short soluble form is also released from the membrane by proteolysis (PubMed:26876177). The sIL11RA is formed either by limited proteolysis of membrane-bound receptors, a process referred to as ectodomain shedding, or directly secreted from the cells after alternative mRNA splicing (PubMed:26876177). mIL11RA is cleaved by the proteases ADAM10, ELANE and PRTN3 (PubMed:26876177)

  • Subunit

    On IL11 binding, forms a multimer complex with IL6ST/gp130

  • SwissProt ID

    Q14626

  • Gene ID
  • Synonyms

    IL11RA; Interleukin-11 receptor subunit alpha; IL-11 receptor subunit alpha; IL-11R subunit alpha; IL-11R-alpha; IL-11RA

  • Research Field

    Immunology

IL-11 Receptor alpha Antibody (YA3092) Related Classifications

MOQ
Minimum order quantity
100 mg

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