Nuclear Matrix Protein p84 Antibody (YA1437)(PBS only)

Cat. No.: HY-P81692A: Data Sheet User Guide for Antibodies
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Nuclear Matrix Protein p84 Antibody (YA1437) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nuclear Matrix Protein p84.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

Nuclear Matrix Protein p84 Antibody (YA1437) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nuclear Matrix Protein p84.

Host

Rabbit

분자량

Predicted band size: 76 kDa;

Observed band size: 84 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q96FV9

Gene ID

9984 [NCBI]

Immunogen

A synthetic peptide of human Nuclear Matrix Protein p84 aa:38-49.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602). Required for efficient export of polyadenylated RNA (PubMed:23222130). The THOC1-THOC2-THOC3 core complex alone is sufficient to bind export factor NXF1-NXT1 and promote ATPase activity of DDX39B/UAP56 (PubMed:33191911). TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed:15833825, PubMed:15998806, PubMed:17190602). Regulates transcriptional elongation of a subset of genes (PubMed:22144908). Involved in genome stability by preventing co-transcriptional R-loop formation (By similarity). May play a role in hair cell formation, hence may be involved in hearing (By similarity); Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1; (Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production

Subcellular Localization：Nucleus speckle; Nucleus, nucleoplasm; Nucleus matrix; Cytoplasm; Cytoplasm

Expression：
Tissue_specificity:It is ubiquitous. It is expressed in a variety of cancer cell lines. Its expression level is extremely low in normal breast epithelial cells, but high in breast tumors. Its expression is closely related to the invasive phenotype of breast tumors, and is also correlated with tumor size and metastatic status.

Induction:Up-regulated during cell proliferation

Isoforms & Post-Translational Modification：Q96FV9 has 2 isomers: Q96FV9-1: 75666 Da (predicted); Q96FV9-2: 43359 Da (predicted).
Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass;Polyubiquitinated, leading to proteasomal degradation; probably involves NEDD4

Subunit：Component of the THO subcomplex, which is composed of THOC1, THOC2, THOC3, THOC5, THOC6 and THOC7 (PubMed:33191911, PubMed:37020021). The THO subcomplex interacts with DDX39B to form the THO-DDX39B complex which multimerizes into a 28-subunit tetrameric assembly (PubMed:33191911, PubMed:37020021). Component of the transcription/export (TREX) complex at least composed of ALYREF/THOC4, DDX39B, SARNP/CIP29, CHTOP and the THO subcomplex; in the complex interacts with THOC2, THOC5 and THOC7 (PubMed:33191911, PubMed:37020021). TREX seems to have a dynamic structure involving ATP-dependent remodeling (PubMed:23222130, PubMed:37020021). Binds to the hypophosphorylated form of RB1. Interacts with RNA polymerase II. Interacts with LUZP4

Research Field

Tags & Cell Markers

Synonyms

Death domain containing protein p84N5; HPR1; hTREX84; Nuclear matrix protein p84; p84N5; THO complex 1; Tho1; Thoc1

각종 서류

User Guide for Antibodies (1077 KB)