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VASP Antibody (YA018)

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Based on 1 Customer Validation

VASP Antibody (YA018) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VASP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for VASP Antibody (YA018)
  • VASP Antibody (YA018) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000-1:2000

Product Details

Description

VASP Antibody (YA018) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VASP.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 45-50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 40 kDa
Species Reactivity Database

Entrez Gene: 7408 Human

SwissProt: P50552 Human

Immunogen

Synthetic peptide corresponding to Human VASP.AA range:51-100/380.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102458

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for VASP Antibody (YA018)
    Western blot analysis of extracts from Hela(lane 2(20μg) or lane 3(40μg)) and MCF-7(lane 4(20μg) or lane 5(40μg)), using VASP (HY-P80368) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80368, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation

  • Subcellular Localization

    Cytoplasm; Cytoplasm, cytoskeleton; Cell junction, focal adhesion; Cell junction, tight junction; Cell projection, lamellipodium membrane; Cell projection, filopodium membrane

  • Expression


    Tissue_specificity:High expression in platelets

  • Subunit

    Homotetramer. Interacts with PFN1, PFN2, LPP, ACTN1 and ACTG1. Interacts, via the EVH1 domain, with the Pro-rich regions of ZYX.

  • SwissProt ID

    P50552

  • Gene ID
    7408 [NCBI]
  • Research Field

    Signal Transduction

VASP Antibody (YA018) Related Classifications

100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    VASP Antibody (YA018) purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg) or lane 3(40μg)) and MCF-7(lane 4(20μg) or lane 5(40μg)), using VASP (HY-P80368) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80368, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • VASP Antibody (YA018) Product Image
    1/16
  • Experimental Validation Results for VASP Antibody (YA018)
  • VASP Antibody (YA018) Product Image
  • VASP Antibody (YA018) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20μg) or lane 3(40μg)) and MCF-7(lane 4(20μg) or lane 5(40μg)), using VASP (HY-P80368) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80368, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • VASP Antibody (YA018) Product Image WB