Albumin Antibody (YA632)

Cat. No.: HY-P80010: Ficha de datos COA
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User Guide for Antibodies
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Albumin Antibody (YA632) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Albumin.

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Tamaño	Stock	Cantidad
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10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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Albumin Antibody (YA632) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Descripciòn

Descripciòn

Albumin Antibody (YA632) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Albumin.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Peso molecular

Predicted band size: 69 kDa;

Observed band size: 69 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P02768

Gene ID

213 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Albumin.AA range:150-190.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200

Sensitivity	Endogenous	Pureza	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

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ALL WB IHC-P

Western blot analysis of extracts from Mouse liver tissue (lane2(20μg), Mouse spleen tissue (lane3(20μg), HepG2 (lane4(20μg) and HEK293 (lane5(20μg) using Albumin Antibody (HY-P80010). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat liver tissue using Albumin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat liver tissue using Albumin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growtH2O2 enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017)

Subcellular Localization：Secreted

Expression：
Tissue_specificity:plasma

Subunit：Interacts with FCGRT; this interaction regulates ALB homeostasis (PubMed:28330995). Interacts with TASOR (By similarity). In plasma, occurs in a covalently-linked complex with cH2O2ophore-bound alpha-1-microglobulin with molar ratio 1:2 and 1:1; this interaction does not prevent fatty acid binding to ALB

RRID

AB_3102799

Database

Entrez Gene: 213 Human ; 11657 Mouse ; 24186 Rat

SwissProt: P02768 Human ; P07724 Mouse ; P02770 Rat

OMIM: 615999 Human

Research Field

Cardiovascular

Documentación

Select Batch:

Ficha de datos (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Albumin Antibody (YA632) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Albumin Antibody (YA632)

Albumin Antibody (YA632) Recomendaciones destacadas:

Product Detail

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Background

Descripciòn

Albumin Antibody (YA632) Related Classifications

Productos vistos recientemente:

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