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TrpC1 Antibody (YA1814)

(Synonyms: HTRP1; Mtrp1; TRP1; TRPC1; Trrp1)
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Based on 1 Customer Validation

TrpC1 Antibody (YA1814) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TrpC1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TrpC1 Antibody (YA1814)
  • TrpC1 Antibody (YA1814) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

TrpC1 Antibody (YA1814) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TrpC1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 120 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 91 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human TRPC1 aa411-492.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TrpC1 Antibody (YA1814)
    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), SH-SY5Y (lane 3), Caco-2 (lane 4), and Mouse brain (lane 5) using TrpC1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    TrpC1 forms a receptor-activated non-selective calcium permeant cation channel. Forms a heteromeric ion channel with TRPC4 or TRPC5 that has reduced calcium permeability compared to the homomeric TRPC4 or TRPC5 channel. Also permeable to monovalent ions including sodium, lithium and cesium ions; Forms a receptor-activated non-selective calcium permeant cation channel. Also activated by intracellular calcium store depletion[1][2][3].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Seems to be ubiquitous

  • Isoforms & Post-Translational Modification

    P48995 has 2 isomers: P48995-1: 91212 Da (predicted); P48995-2: 87617 Da (predicted).
    Activation of PRKCA induces phosphorylation of TRPC1 and subsequent Ca2+ entry into cells

  • Subunit

    Heterotetramer with TRPC4 and/or TRPC5 (PubMed:12032305, PubMed:39478185). Forms a heteromeric ion channel with TRPC4, with a 1:3 TRPC1:TRPC4 stoichiometry (PubMed:39478185). Unlike other TRP channel proteins, does not form a homomeric channel (PubMed:12032305, PubMed:39478185). Interacts with TRPC4AP (By similarity). Interacts with ITPR3 (PubMed:10766822). Interacts with MX1 and RNF24 (PubMed:15757897, PubMed:17850865). Interacts with FKBP4 (PubMed:19945390). Interacts with PLSCR1 (By similarity). Interacts with PKD2L2 (PubMed:16883570). Forms a heterotetramer with PKD2 with a 2:2 stoichiometry; has distinct channel properties separate from PKD2 or TRPC1 homomers alone (PubMed:19193631, PubMed:19850920)

  • SwissProt ID

    P48995

  • Gene ID
    7220 [NCBI]
  • Synonyms

    HTRP1; Mtrp1; TRP1; TRPC1; Trrp1

  • Research Field

    Signal Transduction

References

[1]. Xu SZ, et al. TrpC1 is a membrane-spanning subunit of store-operated Ca(2+) channels in native vascular smooth muscle cells. Circ Res. 2001 Jan 19;88(1):84-7. [Content Brief]

[2]. Ahmmed GU, et al. Protein kinase Calpha phosphorylates the TRPC1 channel and regulates store-operated Ca2+ entry in endothelial cells. J Biol Chem. 2004 May 14;279(20):20941-9. [Content Brief]

[3]. Won J, et al. Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel. Nat Struct Mol Biol. 2025 Feb;32(2):326-338. [Content Brief]

TrpC1 Antibody (YA1814) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), SH-SY5Y (lane 3), Caco-2 (lane 4), and Mouse brain (lane 5) using TrpC1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • TrpC1 Antibody (YA1814) Product Image
    1/16
  • Experimental Validation Results for TrpC1 Antibody (YA1814)
  • TrpC1 Antibody (YA1814) Product Image
  • Experimental Validation Results for TrpC1 Antibody (YA1814)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), SH-SY5Y (lane 3), Caco-2 (lane 4), and Mouse brain (lane 5) using TrpC1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for TrpC1 Antibody (YA1814)
  • TrpC1 Antibody (YA1814) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), SH-SY5Y (lane 3), Caco-2 (lane 4), and Mouse brain (lane 5) using TrpC1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • TrpC1 Antibody (YA1814) Product Image WB