Tyrosine Hydroxylase Antibody (YA023)

MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Tyrosine Hydroxylase Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80362, 1/2000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Tyrosine Hydroxylase Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80362, 1/2000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

MedchemExpress Validation
Flow cytometric analysis of 1X10^6 HEK293 cells labeling Tyrosine Hydroxylase Antibody (HY-P80362, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

MedchemExpress Validation
Immunocytochemistry analysis of C6 cells labeling Tyrosine Hydroxylase with Tyrosine Hydroxylase Antibody (HY-P80362) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tyrosine Hydroxylase Antibody (HY-P80362) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

MedchemExpress Validation
Immunocytochemistry analysis of C6 cells labeling Tyrosine Hydroxylase with Tyrosine Hydroxylase Antibody (HY-P80362) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tyrosine Hydroxylase Antibody (HY-P80362)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Tyrosine Hydroxylase Antibody (YA023) purchased from MedChemExpress. Usage Cited in: Sci Rep. 2026 Feb 16;16(1):9364. [Abstract]

Customer Validation
Effect of treatments on the density of TH-positive neurons in PD rats. NDS exerted a protective effect on dopaminergic neurons. IHC staining for Tyrosine Hydroxylase (TH) in the SN of each group (schematic images for morphological observation). Primary antibody: Tyrosine Hydroxylase Antibody (YA023) (1:1000; incubated overnight).

Tyrosine Hydroxylase Antibody (YA023) purchased from MedChemExpress. Usage Cited in: J Pharmacol Exp Ther. 2025 Dec 8;393(1):103791. [Abstract]

Customer Validation
Immunofluorescence images of TH in the prefrontal cortex (n = 3). Brain tissue was sectioned into 20 μm thick slices using a cryostat. The sections were then incubated at room temperature in a blocking solution containing Triton X-100 for 20 minutes, followed by incubation with primary anti-TH antibody (Tyrosine Hydroxylase Antibody (YA023), HY-P80362) at 4 ◦C for 12 hours. Subsequently, the sections were labeled with the Cy3-conjugated secondary antibodies (Cy3-conjugated Goat Anti-Rabbit IgG H&L, HY-P81017) at room temperature for 2 hours.