1. Cell Cycle/DNA Damage
    Autophagy
    Anti-infection
    Apoptosis
  2. Topoisomerase
    Autophagy
    Mitophagy
    Bacterial
    Apoptosis
    Antibiotic
  3. Etoposide

Etoposide (Synonyms: VP-16; VP-16-213)

Cat. No.: HY-13629 Purity: 99.94%
Handling Instructions

Etoposide (VP-16; VP-16-213) est un agent de chimiothérapie anticancéreuse. Etoposide inhibe la topoisomérase II, il peut arrêter ainsi la réplication de l'ADN. Etoposide induit un arrêt du cycle cellulaire, l'apoptose et l'autophagie.

Etoposid (VP-16; VP-16-213) ist ein Anti-Krebs-Chemotherapeutikum. Etoposid hemmt die topoisomerase II und stoppt so die DNA-Replikation. Etoposid induziert Zellzyklus-Arrest, apoptosis und autophagy.

Etoposide (VP-16; VP-16-213) is an anti-cancer chemotherapy agent. Etoposide inhibits topoisomerase II, thus stopping DNA replication. Etoposide induces cell cycle arrest, apoptosis and autophagy.

For research use only. We do not sell to patients.

Etoposide Chemical Structure

Etoposide Chemical Structure

CAS No. : 33419-42-0

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10 mM * 1 mL in DMSO USD 66 In-stock
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100 mg USD 60 In-stock
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200 mg USD 84 In-stock
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500 mg USD 156 In-stock
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Customer Review

Based on 35 publication(s) in Google Scholar

Other Forms of Etoposide:

Top Publications Citing Use of Products

    Etoposide purchased from MCE. Usage Cited in: Cancer Lett. 2017 Nov 1;408:43-54.

    Western blot analysis of p-p70S6k, p70S6k, p-AKT and AKT after 6 h of treatment with 20 μM RAD001. Levels of p-p70S6k and p-AKT are quantified by densitometric analysis and a corresponding histogram is constructed as relative to p70S6k or AKT and α-tubulin. The lower panel shows a representative Western blot.

    Etoposide purchased from MCE. Usage Cited in: Leuk Lymphoma. 2018 Jan;59(1):162-170.

    WT1 and caspase-3 protein levels are detected in two primary AML blasts treated with 100 μM Eto for 24 h.

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    Description

    Etoposide (VP-16; VP-16-213) is an anti-cancer chemotherapy agent. Etoposide inhibits topoisomerase II, thus stopping DNA replication. Etoposide induces cell cycle arrest, apoptosis and autophagy[1].

    IC50 & Target[1]

    Topoisomerase II

     

    In Vitro

    Etoposide is capable of causing cytotoxicity on pancreatic β-cells by inducing apoptosis through the JNK/ERK-mediated GSK-3 downstream-triggered mitochondria-dependent signaling pathway in RIN-m5F cells[1].
    Etoposide and Anti-Human VEGF significantly abolish P1 sphere-forming ability, an effect associated with apoptosis of this subset of cells[2].
    Etoposide phosphate (0-1μM; 72 hours) inhibits HCT116 FBXW+/+, FBXW-/- and p53-/- as a dose-dependent manner, exhibits IC50s of 0.945 μM; 0.375 μM; and 1.437 μM, respectively[5].
    Etoposide (25 μM; 6 hours) delays p53 recover in FBXW7-deficient cells. In addition, FBXW7 expression is disappeared in FBXW7-/- cells[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[5]

    Cell Line: HCT116 FBXW+/+, FBXW-/- and p53-/- cells
    Concentration: 0.025 μM, 0.05 μM, 0.075 μM, 0.1 μM, 0.2 μM, 0.4 μM, 0.6 μM, 0.8 μM, 1 μM
    Incubation Time: 72 hours
    Result: Inhibits HCT116 FBXW+/+p>, FBXW-/- and p53-/- cell growth as a concentration manner.

    Western Blot Analysis[5]

    Cell Line: HCT116 FBXW7+/+ or FBXW7-/- cells
    Concentration: 25 μM
    Incubation Time: 6 hours
    Result: Exhibited that the recovery of p53 levels after DNA damage is mediated by FBXW7.
    In Vivo

    Etoposide (50 μM) and Anti-Human VEGF-treated hypoxic cells injected intravenously into immunodeficient mice reveals a reduced capacity to induce lung colonies, which also appear with a longer latency period[2]. Etoposide (10 mg/kg/day, i.v.) with NSC 109724 and NSC 241240, reduces the tumor volume in the hepatoblastoma cell injected NMRI nude mice[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Clinical Trial
    Molecular Weight

    588.56

    Formula

    C₂₉H₃₂O₁₃

    CAS No.

    33419-42-0

    SMILES
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 39 mg/mL (66.26 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6991 mL 8.4953 mL 16.9906 mL
    5 mM 0.3398 mL 1.6991 mL 3.3981 mL
    10 mM 0.1699 mL 0.8495 mL 1.6991 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  1% DMSO    99% saline

      Solubility: ≥ 0.5 mg/mL (0.85 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (4.25 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (4.25 mM); Clear solution

    • 4.

      Add each solvent one by one:  5% DMSO    40% PEG300    5% Tween-80    50% saline

      Solubility: ≥ 2.5 mg/mL (4.25 mM); Clear solution

    • 5.

      Add each solvent one by one:  5% DMSO    95% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (4.25 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: 99.94%

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    Keywords:

    EtoposideVP-16 VP-16-213VP16VP 16TopoisomeraseAutophagyMitophagyBacterialApoptosisAntibioticMitochondrial AutophagyHCT116prodrugFBXWp53anti-cancerchemotherapyapoptosisP388leukemiaInhibitorinhibitorinhibit

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