2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. Topoisomerase
  5. DNA gyrase Isoform

DNA gyrase

DNA gyrase is a bacterial type IIA topoisomerase that controls DNA topology by introducing ATP-dependent negative supercoils into DNA[1]. Mechanistically, this activity supports bacterial genome compaction and resolves topological stress during replication, transcription, and cell division[2]. In E. coli, DNA gyrase functions as an A2B2 heterotetramer, in which GyrA and GyrB form coordinated gates for DNA binding, cleavage, strand passage, and religation[2]. Disease-relevant antibacterial research treats DNA gyrase as a validated target because quinolones stabilize gyrase-DNA cleavage complexes, inhibit DNA synthesis, arrest growth, and can lead to chromosome fragmentation[3]. Compared with topoisomerase IV, DNA gyrase remains especially associated with negative supercoiling, whereas topoisomerase IV also contributes to chromosome unlinking after replication[3]. For experimental applications, quinolones, aminocoumarins, and newer bacterial topoisomerase inhibitors provide mechanistic probes for DNA cleavage, ATPase coupling, conformational intermediates, and resistance-linked target mutations[1][2][3]. - DNA gyrase links bacterial DNA supercoiling control with replication, transcription, chromosome architecture, and antibacterial discovery[1][2]. - GyrA/GyrB organization distinguishes gyrase mechanisms from topoisomerase IV in inhibitor-focused studies[2][3]. - Quinolones and NBTIs support structure-guided analysis of cleavage complexes and resistance mechanisms[2][3].

DNA gyrase Related Products (4):

Cat. No. Product Name Effect Purity
  • HY-107044
    DK 507k
    		Modulator
    DK 507k is an orally active 8-methoxyquinolone Antibacterial agent. DK 507k targets DNA gyrase subunit A (GyrA) and modulates the function of GyrA. DK 507k inhibits the growth of various Gram-positive and Gram-negative bacteria, including Pseudomonas aeruginosa and Methicillin (HY-121544)-resistant Staphylococcus aureus. DK 507k eliminates Penicillin-tolerant Streptococcus pneumoniae from the lungs of mice. DK 507k can be used in research related to sepsis and *Streptococcus pneumoniae* pneumonia.
  • HY-178738
    GC-072
    		Inhibitor
    GC-072 is an orally active, 4-oxoquinolizine antibiotic that selectively inhibits bacterial DNA gyrase and Topo IV enzymes. GC-072 does not inhibit human topoisomerases I and II. GC-072 demonstrates strong antimicrobial activity against various bacterial strains, including Gram-positive, Gram-negative, and resistant bacteria. GC-072 also exhibits bactericidal activity against Burkholderia pseudomallei both extracellularly and intracellularly, leading to dose-dependent survival in mice exposed to lethal inhalational models of B. pseudomallei infection. GC-072 can be used for the research of melioidosis.
  • HY-108988
    Albicidin
    		Inhibitor
    Albicidin is a peptide antibiotic with phytotoxic activity. Albicidin potently inhibits bacterial and plant DNA gyrase at nanomolar concentrations, blocks DNA replication, and exhibits excellent antibacterial efficacy against multidrug-resistant bacteria. Albicidin possesses bactericidal activity against Gram-positive and Gram-negative microorganisms, and acts as a virulence factor for the systemic plant infection by Xanthomonas albilineans. Albicidin can be used in studies related to bacterial infections and sugarcane leaf blight.
  • HY-181820
    Topoisomerase IV-IN-3
    		Inhibitor
    Topoisomerase IV-IN-3 is a Staphylococcus aureus Topoisomerase IV inhibitor, DNA gyrase inhibitor and antibacterial agent. Topoisomerase IV-IN-3 has IC50 values of 1.32 μM and 0.48 μM against topoisomerase IV, and 0.88 μM and 0.54 μM against DNA gyrase. Topoisomerase IV-IN-3 inhibits decatenation, ATPase, and supercoiling activities of its target enzymes. Topoisomerase IV-IN-3 exerts antibacterial activity against Gram-positive and Gram-negative bacterial strains. Topoisomerase IV-IN-3 exhibits low cytotoxicity toward human fibroblast cells. Topoisomerase IV-IN-3 can be used for the research of bacterial infections.