Topoisomerase IV-IN-3 

Topoisomerase IV-IN-3 is a Staphylococcus aureus Topoisomerase IV inhibitor, DNA gyrase inhibitor and antibacterial agent. Topoisomerase IV-IN-3 has IC₅₀ values of 1.32 μM and 0.48 μM against topoisomerase IV, and 0.88 μM and 0.54 μM against DNA gyrase. Topoisomerase IV-IN-3 inhibits decatenation, ATPase, and supercoiling activities of its target enzymes. Topoisomerase IV-IN-3 exerts antibacterial activity against Gram-positive and Gram-negative bacterial strains. Topoisomerase IV-IN-3 exhibits low cytotoxicity toward human fibroblast cells. Topoisomerase IV-IN-3 can be used for the research of bacterial infections^[1].

Topoisomerase IV-IN-3 (compound 6b) (0.78-200 μg/mL) potently inhibits Staphylococcus aureus growth with an MIC of 0.78 μg/mL, and shows variable activity against other tested Gram-positive bacterial strains^[1].
Topoisomerase IV-IN-3 (6.25-400 μg/mL) potently inhibits Klebsiella pneumoniae growth with an MIC of 6.25 μg/mL, and shows variable activity against other tested Gram-negative bacterial strains^[1].
Topoisomerase IV-IN-3 (0.48-1.32 μM) potently inhibits Staphylococcus aureus topoisomerase IV ATPase activity with an IC₅₀ of 0.48 μM, and shows inhibitory activity against other tested Staphylococcus aureus DNA gyrase and topoisomerase IV functions^[1].
Topoisomerase IV-IN-3 shows low cytotoxicity against human WI-38 fibroblast cells with an IC₅₀ of 85.56 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

826.78

C₃₄H₄₂N₄O₁₈S

CC(O[C@H]([C@H]([C@@H](O[C@@H]1COC(C)=O)NC(N/N=C/C2=CC=C(C=C2)N=O)=S)OC(C)=O)[C@@H]1O[C@@H]3[C@@H]([C@H]([C@@H]([C@H](O3)COC(C)=O)OC(C)=O)OC(C)=O)OC(C)=O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Van HTK, et al. Maltose-Conjugated Thiosemicarbazones from Substituted Benzaldehydes: Synthesis and Inhibitory Activity against Gram-(+) and Gram-(-) Bacteria. ACS Med Chem Lett. 2026;17(3):670-678. Published 2026 Feb 11.  [Content Brief]