1. Cell Cycle/DNA Damage
  2. Nucleoside Antimetabolite/Analog

5-Fluorouracil (Synonyms: 5-FU)

Cat. No.: HY-90006 Purity: >99.0%
Handling Instructions

5-Fluorouracil is a potent antitumor agent that affects pyrimidine synthesis by inhibiting thymidylate synthetase thus depleting intracellular dTTP pools.

For research use only. We do not sell to patients.

5-Fluorouracil Chemical Structure

5-Fluorouracil Chemical Structure

CAS No. : 51-21-8

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10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
1 g USD 60 In-stock
Estimated Time of Arrival: December 31
5 g USD 84 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Top Publications Citing Use of Products

    5-Fluorouracil purchased from MCE. Usage Cited in: Tumour Biol. 2016 Jul;37(7):8811-24.

    Assays for autophagy activity. Immunoblot analysis for LC3B. Compared with the Lenti-NC cells, the Lenti-CD44v6 cells display a higher intensity of autophagy flux but not steady-state levels when treated with 5-FU (*P<0.001).

    5-Fluorouracil purchased from MCE. Usage Cited in: Int J Biochem Cell Biol. 2018 Mar;96:9-19.

    Hep3B cells are treated with 2 μmol Shikonin, 5-FU, BSNQ or OSNQ for different times (3, 6, 12 and 24 h), and stained with Annexin V and PI. The apoptotic cells are detected by fluorescence microscopy and quantified as percentages.

    5-Fluorouracil purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2018 Jun 27;501(3):636-642.

    Immunoblot analysis of the expression levels of BCL-2 and BAX after treatment of HCT116 TP53-/- cells with 20 μM 5-FU and 1.25 μM DHA alone or in combination for 48 h. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) served as a loading control.

    5-Fluorouracil purchased from MCE. Usage Cited in: Med Sci Monit. 2018 Sep 21;24:6666-6672.

    Expression of protein of OPA1, Mfn1, Mfn2, Drp1 and Fis1 in heart mitochondria of rats treated with different 5-FU concentrations. The control group is intraperitonealled injection with same amount of physiological saline.

    5-Fluorouracil purchased from MCE. Usage Cited in: J Cell Biochem. 2018 Nov 28.

    MKN45 and MKN28 cells transfected with empty and DEC2 vectors are incubated in control medium or 5-Fu of 10 μM for 48 hours, then proliferation ratio of these cells is figured by EdU incorporation assay.

    5-Fluorouracil purchased from MCE. Usage Cited in: J Cell Biochem. 2018 Nov 28.

    The expressions of apoptotic-related proteins, cleaved-PARP, caspase-3-active and survivin are detected in MKN45, MKN45+5-Fu, MKN45-EV, MKN45-DEC2, MKN45-DEC2+5-Fu, MKN28, MKN28+5-Fu, MKN28-EV, MKN28-DEC2, MKN28-DEC2+5-Fu cells using Western blot analysis.

    5-Fluorouracil purchased from MCE. Usage Cited in: J Cell Biochem. 2018 Nov 28.

    The expressions of apoptotic-related proteins, cleaved-PARP, caspase-3-active are detected in control, STAT5A, STAT5A+5-Fu, DEC2+5-Fu, DEC2/STAT5A+5-Fu of MKN45 and MKN28 cell lines using Western blot analysis.
    • Biological Activity

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    • References

    Description

    5-Fluorouracil is a potent antitumor agent that affects pyrimidine synthesis by inhibiting thymidylate synthetase thus depleting intracellular dTTP pools.

    In Vitro

    5-Fluorouracil (5-Fu) and doxorubicin (Dox) show synergistic anticancer efficacy. The IC50 value of 5-Fu/Dox-DNM toward human breast cancer (MDA-MB-231) cells is 0.25 μg/mL, presenting an 11.2-fold and 6.1-fold increase in cytotoxicity compared to Dox-DNM and 5-Fu-DNM, respectively[1]. In 5-fluorouracil (5-FU) and CDDP treated NFBD1-inhibited NPC cells, the NFBD1 expression in NPC CNE1 cell lines is depleted using lentivirus-mediated short hairpin RNA, and the sensitivity of these cells is elevated. NFBD1 knockdown leads to an obvious induction of apoptosis in CDDP- or 5-FU-treated CNE1 cells[3].

    In Vivo

    5-Fluorouracil (23 mg/kg, 3 times/week) for 14 days, induces accelerated gastrointestinal transit associated with acute intestinal inflammation at day 3 after the start of treatment, which may have led to persistent changes in the ENS observed after days 7 and 14 of treatment contributing to delayed gastrointestinal transit and colonic dysmotility[2].

    Clinical Trial
    Solvent & Solubility
    In Vitro: 

    H2O : 16.67 mg/mL (128.15 mM; Need ultrasonic)

    DMSO : 15 mg/mL (115.31 mM; Need ultrasonic and warming)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 7.6876 mL 38.4379 mL 76.8758 mL
    5 mM 1.5375 mL 7.6876 mL 15.3752 mL
    10 mM 0.7688 mL 3.8438 mL 7.6876 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      5-Fluorouracil is dissolved in DMSO and then diluted with PBS[4].

    References
    Animal Administration
    [2]

    Mice receive intraperitoneal injections of 5-FU (23 mg/kg), 3 times a week via a 26 gauge needle. 5-FU is dissolved in 100% dimethyl sulfoxide (DMSO) to make 1 M/L stock solution refrigerated at −20°C. The stock is then defrosted and diluted with sterile water to make 0.1 M/L (10% DMSO) solutions for intraperitoneal injections. The dose of 5-FU is calculated to be equivalent to standard human dose per body surface area. The low doses of 5-FU (10-40 mg/kg) have been shown to have antitumor efficacy in mouse models of cancer. Sham-treated mice received 10% DMSO in sterile water via intraperitoneal injection three times a week via a 26 gauge needle. The injected volumes are calculated to the body weight; the maximum volume does not exceed 200 μL per injection. Mice are euthanized via cervical dislocation at 3 (2 treatments), 7 (3 treatments), and 14 (6 treatments) days after the first injection and colon is collected for in vitro experiments.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    130.08

    Formula

    C₄H₃FN₂O₂

    CAS No.

    51-21-8

    SMILES

    O=C(N1)NC=C(F)C1=O

    Storage

    4°C, protect from light

    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: >99.0%

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    Product Name:
    5-Fluorouracil
    Cat. No.:
    HY-90006
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    5-Fluorouracil

    Cat. No.: HY-90006