Iridoid Glycosides from the Twigs of Sambucus williamsii var. coreana and Their Biological Activities

  • J Nat Prod. 2017 Sep 22;80(9):2502-2508. doi: 10.1021/acs.jnatprod.7b00410.
Won Se Suh  1 ,  Chung Sub Kim  1 ,  Lalita Subedi  2  3 ,  Sun Yeou Kim  2  3 ,  Sang Un Choi  4 ,  Kang Ro Lee  1
Affiliations
  • 1. Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University , Suwon 16419, Republic of Korea.
  • 2. Gachon Institute of Pharmaceutical Science, Gachon University , Incheon 21936, Republic of Korea.
  • 3. College of Pharmacy, Gachon University , #191, Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea.
  • 4. Korea Research Institute of Chemical Technology , Daejeon 34114, Republic of Korea.
PMID: 28841320 DOI: 10.1021/acs.jnatprod.7b00410
Abstract

Six new iridoid glycosides, sambucusides A-F (1-6), and two known derivatives (7 and 8) were isolated from a methanol extract of the twigs of Sambucus williamsii var. coreana. Their chemical structures were elucidated by spectroscopic methods, including NMR (1H and 13C NMR, 1H-1H COSY, HMQC, HMBC, and NOESY) and HRMS. All isolated compounds (1-8) were evaluated for their antiproliferative activities against four human Cancer cell lines (A549, SK-OV-3, SK-MEL-2, and Bt549). Their effects on nitric oxide (NO) production in lipopolysaccharide (LPS)-activated BV-2 cells and their neuroprotective effects through induction of nerve growth factor (NGF) in C6 glioma cells were also examined. Compounds 2, 3, and 5 showed cytotoxic effects (IC50 1.3-8.7 μM) against the SK-MEL-2 and Bt549 cell lines and inhibitory effects on NO production (IC50 of 0.9, 1.3, and 1.2 μM, respectively). Compounds 2, 4, and 8 exhibited NGF-releasing effects (147.0 ± 5.8%, 158.7 ± 5.2%, and 152.6 ± 7.3%, respectively).