Diterpenes from the Trunk of Abies holophylla and Their Potential Neuroprotective and Anti-inflammatory Activities

  • J Nat Prod. 2016 Feb 26;79(2):387-94. doi: 10.1021/acs.jnatprod.5b01053.
Chung Sub Kim  1 ,  Lalita Subedi  2  3 ,  Sun Yeou Kim  2  3 ,  Sang Un Choi  4 ,  Ki Hyun Kim  1 ,  Kang Ro Lee  1
Affiliations
  • 1. Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University , Suwon 16419, Republic of Korea.
  • 2. Gachon Institute of Pharmaceutical Science, Gachon University , Incheon 21936, Republic of Korea.
  • 3. College of Pharmacy, Gachon University , #191, Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea.
  • 4. Korea Research Institute of Chemical Technology , Daejeon 34114, Republic of Korea.
PMID: 26812172 DOI: 10.1021/acs.jnatprod.5b01053
Abstract

Eleven new abietane-type diterpenes, holophyllins D-N (1-11), and 17 known analogues (12-28), were isolated from a MeOH extract of the trunk of Abies holophylla. The chemical structures of 1-11 were determined through spectroscopic data analysis, including NMR ((1)H and (13)C NMR, DEPT, (1)H-(1)H COSY, HMQC, HMBC, and NOESY) and HRFABMS methods. All isolated compounds (1-28) were evaluated for their cytotoxicity against four human tumor cell lines (A549, SK-OV-3, SK-MEL-2, and HCT-116), for their potential neuroprotective effects through induction of nerve growth factor in C6 glioma cells, and for their effects on nitric oxide levels in lipopolysaccharide-stimulated murine microglia BV2 cells.