1. Cell Cycle/DNA Damage
    Autophagy
    Apoptosis
  2. Topoisomerase
    Autophagy
    Apoptosis
  3. Etoposide phosphate

Etoposide phosphate (Synonyms: BMY-40481)

Cat. No.: HY-13630
Handling Instructions

Etoposide phosphate (BMY-40481) is a potent anti-cancer chemotherapy agent and a selective topoisomerase II inhibitor to prevent re-ligation of DNA strands. Etoposide phosphate is the phosphate ester prodrug of etoposide and is considered as active equivalent to Etoposide. Etoposide phosphate induces cell cycle arrest, apoptosis, and autophagy.

For research use only. We do not sell to patients.

Etoposide phosphate Chemical Structure

Etoposide phosphate Chemical Structure

CAS No. : 117091-64-2

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Top Publications Citing Use of Products

    Etoposide phosphate purchased from MCE. Usage Cited in: Cancer Lett. 2017 Nov 1;408:43-54.

    Western blot analysis of p-p70S6k, p70S6k, p-AKT and AKT after 6 h of treatment with 20 μM Everolimus. Levels of p-p70S6k and p-AKT are quantified by densitometric analysis and a corresponding histogram is constructed as relative to p70S6k or AKT and α-tubulin. The lower panel shows a representative Western blot.

    Etoposide phosphate purchased from MCE. Usage Cited in: Leuk Lymphoma. 2018 Jan;59(1):162-170.

    WT1 and caspase-3 protein levels are detected in two primary AML blasts treated with 100 μM Eto for 24 h.

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    Description

    Etoposide phosphate (BMY-40481) is a potent anti-cancer chemotherapy agent and a selective topoisomerase II inhibitor to prevent re-ligation of DNA strands. Etoposide phosphate is the phosphate ester prodrug of etoposide and is considered as active equivalent to Etoposide. Etoposide phosphate induces cell cycle arrest, apoptosis, and autophagy[1][2].

    IC50 & Target[1]

    Topoisomerase II

     

    In Vitro

    Etoposide phosphate is a water-soluble derivative and probable prodrug of etoposide characterized by the presence of a phosphate group in position 4' of the E ring of the etoposide molecule[1].
    Etoposide phosphate (0-1 μM; 72 hours) inhibits HCT116 FBXW+/+, FBXW-/- and p53-/- as a dose-dependent manner, exhibits IC50 values of 0.945 μM; 0.375 μM; and 1.437 μM, respectively[2].
    Etoposide phosphate (25 μM; 6 hours) delays p53 recover in FBXW7-deficient cells. In addition, FBXW7 expression is disappeared in FBXW7-/- cells[2].

    Cell Viability Assay[2]

    Cell Line: FBXW+/+, FBXW-/- and p53-/- cell
    Concentration: 0.025 μM, 0.05 μM, 0.075 μM, 0.1 μM, 0.2 μM, 0.4 μM, 0.6 μM, 0.8 μM, 1 μM
    Incubation Time: 72 hours
    Result: Inhibited HCT116 FBXW+/+, FBXW-/- and p53-/- cell growth as a concentration manner.

    Western Blot Analysis[2]

    Cell Line: HCT116 FBXW7+/+ or FBXW7-/- cells
    Concentration: 25 μM
    Incubation Time: 6 hours
    Result: Exhibited that the recovery of p53 levels after DNA damage is mediated by FBXW7.
    In Vivo

    Etoposide phosphate (intravenous injection; 50, 100, or 150 mg/kg; single dose) has clinical symptomology of progressive ataxia, impaired righting reflex, and splaying and paresis of fore- and hindlimbs at day 8 in female CD-1 mice[3].

    Animal Model: Female CD-1 mice[3]
    Dosage: 50, 100, or 150 mg/kg
    Administration: Intravenous injection; single dose
    Result: Observed degeneration of dorsal root ganglion cells and axonal degeneration of their distal and proximal processes in peripheral nerves, dorsal spinal roots, and dorsal funiculi of the spinal cord at all doses under light microscopy (LM).
    Molecular Weight

    668.54

    Formula

    C₂₉H₃₃O₁₆P

    CAS No.

    117091-64-2

    SMILES

    O=C1OC[[email protected]]2([H])[[email protected]](O[[email protected]]3[[email protected]@H]([[email protected]]([[email protected]@H]([[email protected]@H](CO4)O3)O[[email protected]@H]4C)O)O)C5=C(C=C6OCOC6=C5)[[email protected]@H](C7=CC(OC)=C(OP(O)(O)=O)C(OC)=C7)[[email protected]]21[H]

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.

    References
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    Keywords:

    EtoposideBMY-40481BMY40481BMY 40481TopoisomeraseAutophagyApoptosisHCT116prodrugFBXWp53anti-cancerchemotherapyapoptosisP388leukemiaperipheralneuropathyInhibitorinhibitorinhibit

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