1. Cell Cycle/DNA Damage
    Autophagy
    Apoptosis
  2. Nucleoside Antimetabolite/Analog
    DNA/RNA Synthesis
    Autophagy
    Apoptosis
  3. Gemcitabine

Gemcitabine (Synonyms: LY 188011)

Cat. No.: HY-17026 Purity: 99.92%
Handling Instructions

Gemcitabine (LY 188011) est un pyrimidine nucléoside analogue antimétabolite et un agent antinéoplasique. Gemcitabine inhibe la synthèse de l'ADN et la réparation, entraînant l'autophagie et l'apoptose. Gemcitabine inhibe la croissance des cellules de BxPC-3, de Mia Paca-2, de PANC-1, de PL-45 et de AsPC-1 avec des IC50 de 37,6 nM, 42,9 nM, 92,7 nM, 89,3 nM et 131,4 nM, respectivement.

Gemcitabin (LY 188011) ist ein pyrimidine nucleoside-Antimetabolit und ein antineoplastisches Mittel. Gemcitabin hemmt die DNA synthesis und -Reparatur, was zu autophagy und apoptosis führt. Gemcitabin hemmt das Wachstum von BxPC-3-, Mia Paca-2-, PANC-1-, PL-45- und AsPC-1-Zellen mit IC50s von 37,6 nM, 42,9 nM, 92,7 nM, 89,3 nM bzw. 131,4 nM.

Gemcitabine (LY 188011) is a pyrimidine nucleoside analog antimetabolite and an antineoplastic agent. Gemcitabine inhibits DNA synthesis and repair, resulting in autophagyand apoptosis.

For research use only. We do not sell to patients.

Gemcitabine Chemical Structure

Gemcitabine Chemical Structure

CAS No. : 95058-81-4

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Customer Review

Based on 29 publication(s) in Google Scholar

Other Forms of Gemcitabine:

Top Publications Citing Use of Products

    Gemcitabine purchased from MCE. Usage Cited in: Chem Biol Interact. 2018 Jun 25;290:44-51.

    U2OS and MG-63 cells are treated with Gemcitabine, Licoricidin, or Gemcitabine+Licoricidin for 24 h, followed by the determination of active caspse-3 protein level using western blot. Cells without treatment are used as Control.

    Gemcitabine purchased from MCE. Usage Cited in: J Biol Chem. 2017 Jun 2;292(22):9136-9149.

    The plate clone formation of SW480 and SW620 cells with Gemcitabine (8 nM in SW480 and 16 nM in SW620) and/or PX-12 (4 μM in SW480 and 8 μM in SW620).

    Gemcitabine purchased from MCE. Usage Cited in: Naunyn Schmiedebergs Arch Pharmacol. 2019 May;392(5):615-622.

    The expression level of p-JAK2, p-STAT3, Bcl-XL, and Mcl-1 in PANC-1 cells is detected by western blot analysis and is all down-regulated in the treatment groups.
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    Description

    Gemcitabine (LY 188011) is a pyrimidine nucleoside analog antimetabolite and an antineoplastic agent. Gemcitabine inhibits DNA synthesis and repair, resulting in autophagyand apoptosis[1][2].

    IC50 & Target

    DNA synthesis[1]

    In Vitro

    Gemcitabine (purchased from MedChem Express, 0.003-1 μM; 3 days) kills both mouse and human senescent cells effectively and potently[4].

    Cell Viability Assay[4]

    Cell Line: Non-senescent and replication-induced senescent new born dermal fibroblasts (NBFs)
    Concentration: 0.003, 0.01, 0.03, 0.1, 0.3, 1 μM
    Incubation Time: 3 days
    Result: Killed replication-induced senescent NBFs for 3 days with 11.0% cell viability.
    In Vivo

    Gemcitabine can be administered via endotracheal spray in rats without marked toxicity with a maximum tolerated dose of 4 mg/kg once a week for 9 weeks. The toxicity of Gemcitabine is lower via lung than oral administration at dosages of 2, 4, and 6 mg/kg[2].
    Treatment of the LSL-KrasG12D/+; LSL-Trp53R172H; Pdx-1-Cre mice with either Gemcitabine (50 mg/kg, i.p.) or the combination DMAPT/Gemcitabine significantly increased the median survival time by more than 30 days compared to the placebo group (254.5 [P=0.015] or 255 days [P=0.018] vs. 217.5 days, respectively)[3].

    Clinical Trial
    Molecular Weight

    263.20

    Formula

    C₉H₁₁F₂N₃O₄

    CAS No.

    95058-81-4

    SMILES

    O=C1N(C=CC(N)=N1)[[email protected]]2C(F)([[email protected]@H]([[email protected]](O2)CO)O)F

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 103.3 mg/mL (392.48 mM)

    Ethanol : 3.33 mg/mL (12.65 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7994 mL 18.9970 mL 37.9939 mL
    5 mM 0.7599 mL 3.7994 mL 7.5988 mL
    10 mM 0.3799 mL 1.8997 mL 3.7994 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.58 mg/mL (9.80 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.58 mg/mL (9.80 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.58 mg/mL (9.80 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: 99.92%

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    Keywords:

    GemcitabineLY 188011LY188011LY-188011Nucleoside Antimetabolite/AnalogDNA/RNA SynthesisAutophagyApoptosisInhibitorinhibitorinhibit

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