1. Cell Cycle/DNA Damage
    Autophagy
    Apoptosis
  2. DNA/RNA Synthesis
    Nucleoside Antimetabolite/Analog
    Autophagy
    Apoptosis
  3. Gemcitabine hydrochloride

Gemcitabine hydrochloride (Synonyms: LY 188011 hydrochloride)

Cat. No.: HY-B0003 Purity: 99.87%
Handling Instructions

Gemcitabine Hydrochloride (LY 188011 Hydrochloride) is a pyrimidine nucleoside analog antimetabolite and an antineoplastic agent. Gemcitabine Hydrochloride inhibits DNA synthesis and repair, resulting in autophagyand apoptosis.

For research use only. We do not sell to patients.

Gemcitabine hydrochloride Chemical Structure

Gemcitabine hydrochloride Chemical Structure

CAS No. : 122111-03-9

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Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
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10 mM * 1 mL
ready for reconstitution
USD 66 In-stock
Estimated Time of Arrival: December 31
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100 mg USD 60 In-stock
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200 mg USD 72 In-stock
Estimated Time of Arrival: December 31
500 mg USD 102 In-stock
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1 g USD 120 In-stock
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Customer Review

Based on 44 publication(s) in Google Scholar

Other Forms of Gemcitabine hydrochloride:

Top Publications Citing Use of Products

Publications Citing Use of MCE Gemcitabine hydrochloride

    Gemcitabine hydrochloride purchased from MCE. Usage Cited in: Chem Biol Interact. 2018 Jun 25;290:44-51.

    U2OS and MG-63 cells are treated with Gemcitabine, Licoricidin, or Gemcitabine+Licoricidin for 24 h, followed by the determination of active caspse-3 protein level using western blot. Cells without treatment are used as Control.

    Gemcitabine hydrochloride purchased from MCE. Usage Cited in: J Biol Chem. 2017 Jun 2;292(22):9136-9149.

    The plate clone formation of SW480 and SW620 cells with Gemcitabine (8 nM in SW480 and 16 nM in SW620) and/or PX-12 (4 μM in SW480 and 8 μM in SW620).

    Gemcitabine hydrochloride purchased from MCE. Usage Cited in: Naunyn Schmiedebergs Arch Pharmacol. 2019 May;392(5):615-622.

    The expression level of p-JAK2, p-STAT3, Bcl-XL, and Mcl-1 in PANC-1 cells is detected by western blot analysis and is all down-regulated in the treatment groups.
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    Description

    Gemcitabine Hydrochloride (LY 188011 Hydrochloride) is a pyrimidine nucleoside analog antimetabolite and an antineoplastic agent. Gemcitabine Hydrochloride inhibits DNA synthesis and repair, resulting in autophagyand apoptosis[1][2].

    IC50 & Target

    DNA synthesis[1]

    In Vitro

    Gemcitabine Hydrochloride (purchased from MedChem Express, 0.003-1 μM; 3 days) kills both mouse and human senescent cells effectively and potently[4].
    Gemcitabine Hydrochloride inhibits the growth of BxPC-3, Mia Paca-2, PANC-1, PL-45 and AsPC-1 cells with IC50s of 37.6, 42.9, 92.7, 89.3 and 131.4 nM, respectively[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[4]

    Cell Line: Non-senescent and replication-induced senescent new born dermal fibroblasts (NBFs)
    Concentration: 0.003, 0.01, 0.03, 0.1, 0.3, 1 μM
    Incubation Time: 3 days
    Result: Killed replication-induced senescent NBFs for 3 days with 11.0% cell viability.
    In Vivo

    Gemcitabine Hydrochloride can be administered via endotracheal spray in rats without marked toxicity with a maximum tolerated dose of 4 mg/kg once a week for 9 weeks. The toxicity of Gemcitabine is lower via lung than oral administration at dosages of 2, 4, and 6 mg/kg[2].
    Treatment of the LSL-KrasG12D/+; LSL-Trp53R172H; Pdx-1-Cre mice with either Gemcitabine (50 mg/kg, i.p.) or the combination DMAPT/Gemcitabine Hydrochloride significantly increases the median survival time by more than 30 days compared to the placebo group (254.5 or 255 days vs. 217.5 days, respectively)[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Clinical Trial
    Molecular Weight

    299.66

    Formula

    C₉H₁₂ClF₂N₃O₄

    CAS No.
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 66.66 mg/mL (222.45 mM)

    DMSO : 46.67 mg/mL (155.74 mM; Need ultrasonic)

    DMF : 2.5 mg/mL (8.34 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.3371 mL 16.6856 mL 33.3712 mL
    5 mM 0.6674 mL 3.3371 mL 6.6742 mL
    10 mM 0.3337 mL 1.6686 mL 3.3371 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  PBS

      Solubility: 60 mg/mL (200.23 mM); Clear solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (6.94 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (6.94 mM); Clear solution

    • 4.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (6.94 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: 99.93%

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    Product Name:
    Gemcitabine hydrochloride
    Cat. No.:
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