1. NF-κB
    Immunology/Inflammation
    Metabolic Enzyme/Protease
  2. NF-κB
    Aryl Hydrocarbon Receptor
    E1/E2/E3 Enzyme
  3. Indole-3-carbinol

Indole-3-carbinol (Synonyms: I3C; 3-Indolemethanol)

Cat. No.: HY-N0170 Purity: >98.0%
Handling Instructions

Indole-3-carbinol (I3C) inhibits NF-κB activity and also is an Aryl hydrocarbon receptor (AhR) agonist, and an inhibitor of WWP1 (WW domain-containing ubiquitin E3 ligase 1).

For research use only. We do not sell to patients.

Indole-3-carbinol Chemical Structure

Indole-3-carbinol Chemical Structure

CAS No. : 700-06-1

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
200 mg USD 60 In-stock
Estimated Time of Arrival: December 31
1 g USD 84 In-stock
Estimated Time of Arrival: December 31
5 g   Get quote  
10 g   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 3 publication(s) in Google Scholar

Top Publications Citing Use of Products

Publications Citing Use of MCE Indole-3-carbinol

    Indole-3-carbinol purchased from MCE. Usage Cited in: Molecules. 2018 Oct 11;23(10). pii: E2600.

    The cells are cultured with I3C (50 μM) or 3MC (1 μM) in the presence of IPRN (20 μM). The IPRN-induced inhibition of nucleocytoplasmic shuttling of AhR is suppressed by I3C and 3MC.

    Indole-3-carbinol purchased from MCE. Usage Cited in: Molecules. 2018 Oct 11;23(10). pii: E2600.

    The cells are cultured with I3C (50 μM) or 3MC (1 μM) in the presence of IPRN (20 μM). The mRNA expression of CYP1A1 is increased after the cotreatment of I3C and 3MC.

    View All NF-κB Isoform Specific Products:

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Indole-3-carbinol (I3C) inhibits NF-κB activity and also is an Aryl hydrocarbon receptor (AhR) agonist, and an inhibitor of WWP1 (WW domain-containing ubiquitin E3 ligase 1).

    IC50 & Target[1][2]

    NF-κB

     

    AhR

     

    In Vitro

    It is found that Indole-3-carbinol (I3C) inhibits the proliferation of THP-1 cells in a dose- and time dependent manner with minimal toxicity over normal monocytes. The AhR target genes (CYP1A1, IL1β) are overexpressed upon Indole-3-carbinol treatment (p<0.05 to p<0.001). The antiproliferative effects of Indole-3-carbinol are in association with programing cell death. Indole-3-carbinol downregulates BCL2 and upregulates FasR in THP-1 cells (p<0.05 to p<0.001). G1 cell cycle arrest is also observed using flow cytometry. G1-acting cell cycle genes (P21, P27 and P53) are overexpressed (p<0.05 to p<0.001), while CDK2 is downregulated upon Indole-3-carbinol treatment (p<0.01 to p<0.001)[1].Indole-3-carbinol suppresses NF-κB activity and stimulates the p53 pathway in pre-B acute lymphoblastic leukemia cells[2].

    Clinical Trial
    Molecular Weight

    147.17

    Formula

    C₉H₉NO

    CAS No.

    700-06-1

    SMILES

    OCC1=CNC2=C1C=CC=C2

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 150 mg/mL (1019.23 mM; Need ultrasonic and warming)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.7949 mL 33.9743 mL 67.9486 mL
    5 mM 1.3590 mL 6.7949 mL 13.5897 mL
    10 mM 0.6795 mL 3.3974 mL 6.7949 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay
    [1]

    THP-1 cells are cultured in RPMI 1640 supplemented with 10% FBS, 100 U/mL penicillin, 100 mg/mL streptomycin and 2 mM Glutamax in a fully humidified atmosphere with 5% CO2. Cells (2-5×105 mL-1) are seeded in six well plates followed by resuspension in complete growth media. THP-1 monocyte cells are then treated with 10 ng/mL phorbol 12-myristate 13-acetate as a tumor promoter to induce stable differentiation into attaching macrophage-like cells and overexpression of AhR. The cells are then treated with varying concentrations of Indole-3-carbinol (1, 10 and 100 μM, and 1 mM). THP-1 cells and enriching normal monocytes are seeded at 5×104 cells/well in 24-well plate with different concentrations of Indole-3-carbinol and observed for 24 and 48 h followed by MTT assay. The cells are incubated in triplicates in a final volume of 200 mL of Phenol Red free RPMI 1640 for 20 h . An aliquot of 20 mL of MTT solution (5 mg/mL) is added to each well and incubated for 4 h. Formazan crystals are formed. An amount of 300 mL DMSO is then added to each well as a cell lysis solution. Percentage of cell viability is assessed by spectrophotometry at 570 nm[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.
    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Keywords:

    Indole-3-carbinolI3C3-IndolemethanolNF-κBAryl Hydrocarbon ReceptorE1/E2/E3 EnzymeNuclear factor-κBNuclear factor-kappaBAhRE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product name

     

    Salutation

    Applicant name *

     

    Email address *

    Phone number *

     

    Organization name *

    Country or Region *

     

    Requested quantity *

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product name:
    Indole-3-carbinol
    Cat. No.:
    HY-N0170
    Quantity:
    MCE Japan Authorized Agent: