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NMDAR1 Antibody (YA7361)

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Based on 1 Customer Validation

NMDAR1 Antibody (YA7361) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to NMDAR1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG,Kappa

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NMDAR1 Antibody (YA7361)
  • NMDAR1 Antibody (YA7361) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000

Product Details

Description

NMDAR1 Antibody (YA7361) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to NMDAR1.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 120 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 105 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG,Kappa

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NMDAR1 Antibody (YA7361)
    Western blot analysis of extracts from Human brain cancer (lane 1(20μg)) 、Mouse brain (lane 2(20μg)) 、unboiled Mouse brain (lane 3(20μg)) 、Rat brain (lane 4(20μg)) and unboiled Rat brain (lane 5(20μg)) using NMDAR1 Antibody (HY-P87676) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    NMDAR1 component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the long-term potentiation (LTP). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium. Each GluN2 or GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15].

  • Subcellular Localization

    Cell membrane,Postsynaptic cell membrane,Postsynaptic density membrane,Synaptic cell membrane

  • Isoforms & Post-Translational Modification

    Q05586 has 7 isomers: Q05586-1: 105373 Da (predicted); Q05586-2: 99315 Da (predicted); Q05586-3: 101209 Da (predicted); Q05586-4: 103479 Da (predicted); Q05586-5: 107909 Da (predicted); Q05586-6: 106015 Da (predicted); Q05586-7: 101851 Da (predicted).
    NMDA is probably regulated by C-terminal phosphorylation of an isoform of GRIN1 by PKC (PubMed:8316301)

  • Subunit

    Heterotetramer; the NMDAR subunits are modular and harbor tiered domains that function in concert to regulate opening and closing of the cation-selective ion channel pore (PubMed:36309015, PubMed:38598639)

  • SwissProt ID

    Q05586

NMDAR1 Antibody (YA7361) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Human brain cancer (lane 1(20μg)) 、Mouse brain (lane 2(20μg)) 、unboiled Mouse brain (lane 3(20μg)) 、Rat brain (lane 4(20μg)) and unboiled Rat brain (lane 5(20μg)) using NMDAR1 Antibody (HY-P87676) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • NMDAR1 Antibody (YA7361) Product Image
    1/16
  • Experimental Validation Results for NMDAR1 Antibody (YA7361)
  • NMDAR1 Antibody (YA7361) Product Image
  • Experimental Validation Results for NMDAR1 Antibody (YA7361)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Human brain cancer (lane 1(20μg)) 、Mouse brain (lane 2(20μg)) 、unboiled Mouse brain (lane 3(20μg)) 、Rat brain (lane 4(20μg)) and unboiled Rat brain (lane 5(20μg)) using NMDAR1 Antibody (HY-P87676) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NMDAR1 Antibody (YA7361)
  • NMDAR1 Antibody (YA7361) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Human brain cancer (lane 1(20μg)) 、Mouse brain (lane 2(20μg)) 、unboiled Mouse brain (lane 3(20μg)) 、Rat brain (lane 4(20μg)) and unboiled Rat brain (lane 5(20μg)) using NMDAR1 Antibody (HY-P87676) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • NMDAR1 Antibody (YA7361) Product Image WB