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P2RY12 Antibody (YA3135)(PBS only)

(Synonyms: HORK3; P2Y12; ADPG-R; BDPLT8; SP1999; P2T(AC); P2Y(AC); P2Y(12)R; P2Y(ADP); P2Y(cyc))

P2RY12 Antibody (YA3135) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to P2RY12.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for P2RY12 Antibody (YA3135)(PBS only)
  • P2RY12 Antibody (YA3135)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

P2RY12 Antibody (YA3135) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to P2RY12.

  • Host Rabbit
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 52 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 39 kDa
Immunogen

A synthetic peptide of human P2Y12

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for P2RY12 Antibody (YA3135)(PBS only)
    Western blot analysis of extracts from A549 (lane 2(20μg), HL-60 (lane 3(20μg), U251 (lane 4(20μg), using P2Y12 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    P2RY12 receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Required for normal platelet aggregation and blood coagulation

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:It is highly expressed in platelets and less so in brain tissue. It is expressed at the lowest levels in the lungs, appendix, pituitary gland, and adrenal glands. It is also expressed in the spinal cord and fetal brain tissue.

  • SwissProt ID

    Q9H244

  • Gene ID
    64805 [NCBI]
  • Synonyms

    HORK3; P2Y12; ADPG-R; BDPLT8; SP1999; P2T(AC); P2Y(AC); P2Y(12)R; P2Y(ADP); P2Y(cyc)

  • Research Field

    Neuroscience

P2RY12 Antibody (YA3135)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2(20μg), HL-60 (lane 3(20μg), U251 (lane 4(20μg), using P2Y12 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • P2RY12 Antibody (YA3135)(PBS only) Product Image
    1/16
  • Experimental Validation Results for P2RY12 Antibody (YA3135)(PBS only)
  • P2RY12 Antibody (YA3135)(PBS only) Product Image
  • Experimental Validation Results for P2RY12 Antibody (YA3135)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2(20μg), HL-60 (lane 3(20μg), U251 (lane 4(20μg), using P2Y12 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for P2RY12 Antibody (YA3135)(PBS only)
  • P2RY12 Antibody (YA3135)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane 2(20μg), HL-60 (lane 3(20μg), U251 (lane 4(20μg), using P2Y12 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • P2RY12 Antibody (YA3135)(PBS only) Product Image WB