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hnRNP M Antibody (YA3034)(PBS only)

(Synonyms: CEAR; HNRPM; HTGR1; NAGR1; HNRPM4; HNRNPM4; hnRNP M)

hnRNP M Antibody (YA3034) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to hnRNP M.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for hnRNP M Antibody (YA3034)(PBS only)
  • hnRNP M Antibody (YA3034)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Description

hnRNP M Antibody (YA3034) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to hnRNP M.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 78 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 78 kDa
Immunogen

A synthetic peptide of human hnRNP M1-M4

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for hnRNP M Antibody (YA3034)(PBS only)
    Western blot analysis was performed on extracts from HeLa (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Jurkat (lane 3, 15 μg), and 293T (lane 4, 15 μg) using hnRNP M Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    hnRNP M is a Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines

  • Subcellular Localization

    Nucleus, nucleolus

  • Isoforms & Post-Translational Modification

    P52272 has 3 isomers: P52272-1: 77516 Da (predicted); P52272-2: 73621 Da (predicted); P52272-3: Da (predicted).
    Sumoylated

  • Subunit

    Identified in the spliceosome C complex (PubMed:11991638). Interacts with PPIA/CYPA (PubMed:25678563)

  • SwissProt ID

    P52272

  • Gene ID
    4670 [NCBI]
  • Synonyms

    CEAR; HNRPM; HTGR1; NAGR1; HNRPM4; HNRNPM4; hnRNP M

  • Research Field

    Tags & Cell Markers

hnRNP M Antibody (YA3034)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HeLa (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Jurkat (lane 3, 15 μg), and 293T (lane 4, 15 μg) using hnRNP M Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • hnRNP M Antibody (YA3034)(PBS only) Product Image
    1/16
  • Experimental Validation Results for hnRNP M Antibody (YA3034)(PBS only)
  • hnRNP M Antibody (YA3034)(PBS only) Product Image
  • Experimental Validation Results for hnRNP M Antibody (YA3034)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HeLa (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Jurkat (lane 3, 15 μg), and 293T (lane 4, 15 μg) using hnRNP M Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for hnRNP M Antibody (YA3034)(PBS only)
  • hnRNP M Antibody (YA3034)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HeLa (lane 1, 15 μg), HepG2 (lane 2, 15 μg), Jurkat (lane 3, 15 μg), and 293T (lane 4, 15 μg) using hnRNP M Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • hnRNP M Antibody (YA3034)(PBS only) Product Image WB