P Glycoprotein Antibody (YA255)(PBS only)

Cat. No.: HY-P80772A: Ficha de datos User Guide for Antibodies
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P Glycoprotein Antibody (YA255) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to P Glycoprotein.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

P Glycoprotein Antibody (YA255) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to P Glycoprotein.

Host

Rabbit

Peso molecular

Predicted band size: 141 kDa;

Observed band size: 130-180 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P08183

Gene ID

5243 [NCBI]

Immunogen

Synthetic peptide corresponding to Human P Glycoprotein.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:50000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:1200

Pureza	affinity purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：Translocates drugs and phospholipids across the membrane (PubMed:2897240, PubMed:35970996, PubMed:8898203, PubMed:9038218, PubMed:35507548). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:35970996, PubMed:9038218)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Apical cell membrane; Cytoplasm

Expression：
Tissue_specificity:It is expressed in the small intestine (PubMed: 28408210) . It is also expressed in the liver, kidneys, and brain.

Isoforms & Post-Translational Modification：P08183 has 2 isomers: P08183-1: 141479 Da (predicted); P08183-2: 134032 Da (predicted).

Subunit：Interacts with PSMB5. Finds in a complex with ABCB1, TFPI2 and PPP2R3C; leading to the dephosphorylation of ABCB1

Research Field

Signal Transduction

Synonyms

p-pg; PGP; ABCB1; MDR1; PGY1; Multidrug resistance protein 1; ATP-binding cassette sub-family B member 1; P-glycoprotein 1; CD antigen CD243

Documentación

User Guide for Antibodies (1077 KB)