Toll-Like Receptor 3 Antibody

Cat. No.: HY-P80917: Data Sheet COA
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User Guide for Antibodies
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Toll-Like Receptor 3 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Toll-Like Receptor 3.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Toll-Like Receptor 3 Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Toll-Like Receptor 3 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Toll-Like Receptor 3.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 105 kDa;

Observed band size: 130 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O15455

Gene ID

7098 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human TLR3: 801-905/905.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-10000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from K562 (lane 1) and Hela (lane 2) and M-Intestine (lane 3) using TLR3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

Function：Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response

Subcellular Localization：Endoplasmic reticulum membrane; Single-pass type I membrane protein; Endosome membrane; Early endosome

Expression：
Tissue_specificity:It is highly expressed in the placenta and pancreas. It can also be detected in CD11c+ immature dendritic cells. It is expressed only in dendritic cells and not in other leukocytes (including monocyte precursors) . TLR3 is the most strongly expressed TLR in the brain, especially in astrocytes, glial cells, and neurons.

Isoforms & Post-Translational Modification：O15455 has 2 isomers: O15455-1: 103829 Da (predicted); O15455-2: 72937 Da (predicted).
Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding;TLR3 signaling requires a proteolytic cleavage mediated by cathepsins CTSB and CTSH, the cleavage occurs between amino acids 252 and 346. The cleaved form of TLR3 is the predominant form found in endosomes;Ubiquitinated by TRIM3; leading to recognition and sorting of polyubiquitinated TLR3 by the ESCRT complexes (PubMed:32878999). Ubiquitinated by ZNRF1 via 'Lys-63'-linked ubiquitin chains; leading to TLR3 lysosomal trafficking and degradation (PubMed:37158982). Ubiquitinated by RNF170 at Lys-765 via 'Lys-48'-linked ubiquitin chains; leading to TLR3 proteasomal degradation (PubMed:31076723)

Subunit：Monomer and homodimer; dimerization is triggered by ligand-binding, the signaling unit is composed of one ds-RNA of around 40 bp and two TLR3 molecules, and lateral clustering of signaling units along the length of the ds-RNA ligand is required for TLR3 signal transduction. Interacts (via transmembrane domain) with UNC93B1; the interaction is required for transport from the ER to the endosomes (PubMed:33432245). Interacts with SRC; upon binding of double-stranded RNA. Interacts with TICAM1 (via the TIR domain) in response to poly(I:C) and this interaction is enhanced in the presence of WDFY1 (PubMed:25736436). The tyrosine-phosphorylated form (via TIR domain) interacts with WDFY1 (via WD repeat 2) in response to poly(I:C) (PubMed:25736436)

RRID

AB_3102254

Database

Entrez Gene: 7098 Human ; 142980 Mouse ;

SwissProt: O15455 Human ; Q99MB1 Mouse ;

OMIM: 613002 Human

Research Field

Immunology

Synonyms

CD283; CD283 antigen; IIAE2; TLR 3; Tlr3; TLR3_HUMAN; Toll Like Receptor 3; Toll-like receptor 3.

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Toll-Like Receptor 3 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Toll-Like Receptor 3 Antibody

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Background

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Toll-Like Receptor 3 Antibody Related Classifications

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