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KDM4B Antibody (YA2536)

(Synonyms: KDM4B; JHDM3B; JMJD2B; KIAA0876; Lysine-specific demethylase 4B; JmjC domain-containing histone demethylation protein 3B; Jumonji domain-containing protein 2B)
Customer Review

Based on 1 Customer Validation

KDM4B Antibody (YA2536) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KDM4B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • KDM4B Antibody (YA2536) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

KDM4B Antibody (YA2536) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KDM4B.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 150 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 122 kDa
Species Reactivity Database

Entrez Gene: 23030 Human ;

SwissProt: O94953 Human ;

Immunogen

A synthesized peptide derived from human KDM4B / JMJD2B aa100-144.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104887

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KDM4B Antibody (YA2536)
    Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), SW480 (lane 2, 15 μg), Hela (lane 3, 15 μg), HCT116 (lane 4, 15 μg), Mouse testis (lane 5, 15 μg), and Rat testis (lane 6, 15 μg) using KDM4B Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for KDM4B Antibody (YA2536)
    Western blot analysis of extracts from RAW264.7(lane 2(20ug)，HCT116(lane 3(20ug) and HEK293(lane 4(20ug) using KDM4B Antibody (HY-P82791) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for KDM4B Antibody (YA2536)
    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using KDM4B Antibody (YA2536). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82791, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for KDM4B Antibody (YA2536)
    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using KDM4B Antibody (YA2536). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82791, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for KDM4B Antibody (YA2536)
    Immunocytochemistry analysis of Hela cells labeling KDM4B Antibody (HY-P82791) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with KDM4B Antibody (HY-P82791) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for KDM4B Antibody (YA2536)
    Immunocytochemistry analysis of Hela cells labeling KDM4B Antibody (HY-P82791) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with KDM4B Antibody (HY-P82791) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    KDM4B is a Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate. Plays a critical role in the development of the central nervous system (CNS)[1][2].

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    O94953 has 2 isomers: O94953-1: 121897 Da (predicted); O94953-2: 50582 Da (predicted).

  • SwissProt ID

    O94953

  • Gene ID
    23030 [NCBI]
  • Synonyms

    KDM4B; JHDM3B; JMJD2B; KIAA0876; Lysine-specific demethylase 4B; JmjC domain-containing histone demethylation protein 3B; Jumonji domain-containing protein 2B

  • Research Field

    Epigenetics and Nuclear Signaling

KDM4B Antibody (YA2536) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), SW480 (lane 2, 15 μg), Hela (lane 3, 15 μg), HCT116 (lane 4, 15 μg), Mouse testis (lane 5, 15 μg), and Rat testis (lane 6, 15 μg) using KDM4B Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug)，HCT116(lane 3(20ug) and HEK293(lane 4(20ug) using KDM4B Antibody (HY-P82791) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using KDM4B Antibody (YA2536). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82791, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using KDM4B Antibody (YA2536). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82791, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling KDM4B Antibody (HY-P82791) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with KDM4B Antibody (HY-P82791) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling KDM4B Antibody (HY-P82791) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with KDM4B Antibody (HY-P82791) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • KDM4B Antibody (YA2536) Product Image
    1/16
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • KDM4B Antibody (YA2536) Product Image
  • Experimental Validation Results for KDM4B Antibody (YA2536)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), SW480 (lane 2, 15 μg), Hela (lane 3, 15 μg), HCT116 (lane 4, 15 μg), Mouse testis (lane 5, 15 μg), and Rat testis (lane 6, 15 μg) using KDM4B Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for KDM4B Antibody (YA2536)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from RAW264.7(lane 2(20ug)，HCT116(lane 3(20ug) and HEK293(lane 4(20ug) using KDM4B Antibody (HY-P82791) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for KDM4B Antibody (YA2536)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using KDM4B Antibody (YA2536). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82791, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for KDM4B Antibody (YA2536)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using KDM4B Antibody (YA2536). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82791, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for KDM4B Antibody (YA2536)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling KDM4B Antibody (HY-P82791) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with KDM4B Antibody (HY-P82791) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for KDM4B Antibody (YA2536)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling KDM4B Antibody (HY-P82791) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with KDM4B Antibody (HY-P82791) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • Experimental Validation Results for KDM4B Antibody (YA2536)
  • KDM4B Antibody (YA2536) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from A549 (lane 1, 15 μg), SW480 (lane 2, 15 μg), Hela (lane 3, 15 μg), HCT116 (lane 4, 15 μg), Mouse testis (lane 5, 15 μg), and Rat testis (lane 6, 15 μg) using KDM4B Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • KDM4B Antibody (YA2536) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from RAW264.7(lane 2(20ug)，HCT116(lane 3(20ug) and HEK293(lane 4(20ug) using KDM4B Antibody (HY-P82791) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • KDM4B Antibody (YA2536) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using KDM4B Antibody (YA2536). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82791, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • KDM4B Antibody (YA2536) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using KDM4B Antibody (YA2536). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82791, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • KDM4B Antibody (YA2536) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling KDM4B Antibody (HY-P82791) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with KDM4B Antibody (HY-P82791) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • KDM4B Antibody (YA2536) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling KDM4B Antibody (HY-P82791) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with KDM4B Antibody (HY-P82791) at 1/200 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • KDM4B Antibody (YA2536) Product Image WB
  • KDM4B Antibody (YA2536) Product Image WB
  • KDM4B Antibody (YA2536) Product Image IHC-P
  • KDM4B Antibody (YA2536) Product Image IHC-P
  • KDM4B Antibody (YA2536) Product Image ICC
  • KDM4B Antibody (YA2536) Product Image ICC