Cyclin B1 Antibody (YA4805)
(Synonyms: CCNB1; CCNB; G2/mitotic-specific cyclin-B1)Cyclin B1 Antibody (YA4805) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Cyclin B1.
-
Host:
Mouse
-
Application:
WB, IP, ICC/IF, FC, ELISA
-
Reactivity :
Human, Rat
-
Formulation:
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
|
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
|
|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
Cyclin B1 Antibody (YA4805) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Cyclin B1.
-
Host Mouse
-
Clonality Monoclonal
-
Species ReactivityHuman, Rat
-
Calculated Molecular Weight Predicted band size: 48 kDa;
Purified recombinant fragment of human Cyclin B1 expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Cyclin B1 is essential for the control of the cell cycle at the G2/M (mitosis) transition
-
Subcellular Localization
Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
-
Isoforms & Post-Translational Modification
P14635 has 2 isomers: P14635-1: 48337 Da (predicted); P14635-2: 44102 Da (predicted).
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction (PubMed:16009132). Deubiquitinated by USP22 during G2/M phase (PubMed:27030811);Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site -
Subunit
Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization. Interacts with CDK5RAP3 (PubMed:15790566). Interacts with RFPL4A and UBE2A (By similarity). Interacts with INCA1 (PubMed:21540187)
-
SwissProt ID
-
Synonyms
CCNB1; CCNB; G2/mitotic-specific cyclin-B1
Documentation
Powered by Bioz
