ASCC2 Antibody (YA7978)(PBS only)

Cat. No.: HY-P88294A: Data Sheet User Guide for Antibodies
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ASCC2 Antibody (YA7978) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ASCC2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

ASCC2 Antibody (YA7978) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ASCC2.

Host

Mouse

Molecular Weight

Predicted band size: 86.2 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9H1I8

Immunogen

Full length human recombinant protein of human ASCC2 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Ubiquitin-binding protein involved in DNA repair and rescue of stalled ribosomes (PubMed:29144457, PubMed:32099016, PubMed:32579943, PubMed:36302773). Plays a role in DNA damage repair as component of the ASCC complex (PubMed:29144457). Recruits ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains (PubMed:29144457). Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation (PubMed:12077347). Involved in activation of the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation (PubMed:32099016, PubMed:32579943, PubMed:36302773). Specifically recognizes and binds RPS20/uS10 ubiquitinated by ZNF598, promoting recruitment of the RQT (ribosome quality control trigger) complex on stalled ribosomes, followed by disassembly of stalled ribosomes (PubMed:36302773)

Subcellular Localization：Nucleus,Nucleus speckle

Expression：
Tissue_Specificity: Ubiquitous

Isoforms & Post-Translational Modification：Q9H1I8 has three isomers: Q9H1I8-1: 86360 Da (predicted); Q9H1I8-2: 41794 Da (predicted); Q9H1I8-3: 77369 Da (predicted).

Subunit：Identified in the ASCC complex that contains ASCC1, ASCC2 and ASCC3 (PubMed:29144457, PubMed:29997253)

Synonyms

ASC1p100; p100

Documentation

User Guide for Antibodies (1077 KB)