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TRIM21 Antibody (YA658)

(Synonyms: SSA; RO52; SSA1; RNF81; Ro/SSA)
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Based on 1 Customer Validation

TRIM21 Antibody (YA658) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TRIM21.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TRIM21 Antibody (YA658)
  • TRIM21 Antibody (YA658) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

TRIM21 Antibody (YA658) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TRIM21.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Species Reactivity Database

Entrez Gene: 6737 Human

SwissProt: P19474 Human

Immunogen

Synthetic peptide corresponding to Human TRIM21.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102753

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TRIM21 Antibody (YA658)
    Western blot analysis of extracts from HEK293T (lane2(20μg), MOLT-4 (lane3(20μg), HeLa (lane4(20μg) and A549 (lane5(20μg) using TRIM21 Antibody (HY-P80921). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Tripartite motif-containing 21 (TRIM21) is a cytosolic E3 ubiquitin ligase that functions in intracellular immunity and protein homeostasis[1][2]. Mechanistically, TRIM21 mediates K48- and K11-linked ubiquitination of target proteins, including viral antigens and transcription factors, modulating their stability, signaling, and degradation[3][4][5]. TRIM21 regulates protein translation by interacting with PKR and promoting K6-linked ubiquitination of PP1α, thereby controlling eIF2α dephosphorylation and protein synthesis under stress conditions[6]. In cancer models, TRIM21 suppresses tumorigenesis by promoting cuproptosis in esophageal squamous cell carcinoma through ID1 ubiquitination and inhibits ovarian cancer cell proliferation and migration via cell cycle regulation[3][7]. TRIM21 also contributes to hepatocarcinogenesis by negatively regulating the p62-Keap1-Nrf2 antioxidant pathway, enhancing oxidative stress and tumor progression[8]. Compared with related TRIM isoforms, TRIM21 specifically targets interferon regulatory factors, such as IRF3 and IRF5, with differential isoform-specific degradation, thereby modulating innate immunity and autoimmune responses[9][10]. Experimental applications exploit TRIM21’s antibody-binding Fc domain to degrade specific intracellular proteins, enabling precise protein depletion in cell and zebrafish models[1][11]. Small molecules or inhibitors targeting upstream regulators, like Sorafenib, can enhance TRIM21-mediated ubiquitination, highlighting its potential in therapeutic intervention and functional studies[3][5]. Collectively, TRIM21 integrates immune defense, protein quality control, and disease modulation, making it a versatile tool for mechanistic and translational research.

  • Subcellular Localization

    Cytoplasm; Cytoplasmic vesicle, autophagosome; Nucleus; Cytoplasm, P-body; Cytoplasm, Stress granule

  • Expression


    Tissue_specificity:Isoform 1 and isoform 2 are expressed in human heart and fetal lung

    Induction:Up-regulated by isoform 2 of XBP1. Up-regulated by IFNG/interferon-gamma, with a peak after 2-4 hours of treatment in monocytes/macrophages

  • Isoforms & Post-Translational Modification

    P19474 has 2 isomers: P19474-1: 54170 Da (predicted); P19474-2: 45057 Da (predicted).
    Autoubiquitinated; does not lead to its proteasomal degradation. Deubiquitinated by USP4; leading to its stabilization

  • Subunit

    Homotrimer (PubMed:17156811, PubMed:26347139). Interacts (via C-terminus) with IRF8 (via C-terminus) (By similarity). Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Interacts with CALR, CUL1, FBXW11, HSPA5, IKBKB, IRF3, SKP1 and VCP. Interacts with SKP2; the interaction with SKP2 does not depend on an intact F-box domain. Interacts (via N-terminus and C-terminus) with DCP2 (via N-terminus and C-terminus). Interacts with ULK1, BECN1 and with ATG8 family members, including GABARAP, GABARAPL1, GABARAPL2 and MAP1LC3C/LC3C. Interacts with TRIM21 and SQSTM1/sequestosome 1. Interacts with IRF3 (By similarity) (PubMed:12699405, PubMed:16880511, PubMed:17156811, PubMed:18022694, PubMed:18361920, PubMed:18641315, PubMed:19675099, PubMed:26347139, PubMed:8666824). Interacts (via the SPRY domain) with NMI (via coiled-coil domain); the interaction promotes 'Lys-63'-linked ubiquitination of NMI (PubMed:26342464). Interacts with IFI35 and NMI; the interaction facilitates NMI-IFI35 complex formation (PubMed:26342464)

  • SwissProt ID

    P19474

  • Gene ID
    6737 [NCBI]
  • Synonyms

    SSA; RO52; SSA1; RNF81; Ro/SSA

  • Research Field

    Epigenetics and Nuclear Signaling

[1]. Foss S, et al. TRIM21-From Intracellular Immunity to Therapy. Front Immunol. 2019 Aug 28;10:2049. [Content Brief]

[2]. Jones EL, et al. TRIM21/Ro52 - Roles in Innate Immunity and Autoimmune Disease. Front Immunol. 2021 Sep 6;12:738473. [Content Brief]

[3]. Li L, et al. TRIM21-Mediated K11-Linked Ubiquitination of ID1 Suppresses Tumorigenesis and Promotes Cuproptosis in Esophageal Squamous Cell Carcinoma. Adv Sci (Weinh). 2025 Sep;12(35):e02501. [Content Brief]

[4]. Wu S, et al. Ubiquitin-dependent proteasomal degradation of small hepatitis B virus surface antigen mediated by TRIM21 and antagonized by OTUD4. J Virol. 2025 May 20;99(5):e0230924. [Content Brief]

[5]. Li Y, et al. CNOT7 facilitates radiation resistance in colorectal cancer through TRIM21/XRCC6-mediated non-homologous end joining repair. Cell Death Dis. 2025 Nov 17;16(1):833. [Content Brief]

[6]. Hermans A, et al. A 3D-Printed and Freely Available Device to Measure the Zebrafish Optokinetic Response Before and After Injury. Zebrafish. 2024 Apr;21(2):144-148. [Content Brief]

[7]. Sun J, et al. TRIM21 deficiency promotes cell proliferation and tumorigenesis via regulating p21 expression in ovarian cancer. Bioengineered. 2022 Mar;13(3):6024-6035. [Content Brief]

[8]. Wang F, et al. The Ubiquitin E3 Ligase TRIM21 Promotes Hepatocarcinogenesis by Suppressing the p62-Keap1-Nrf2 Antioxidant Pathway. Cell Mol Gastroenterol Hepatol. 2021;11(5):1369-1385. [Content Brief]

[9]. Stacey KB, et al. Tyrosine phosphorylation of the E3 ubiquitin ligase TRIM21 positively regulates interaction with IRF3 and hence TRIM21 activity. PLoS One. 2012;7(3):e34041. [Content Brief]

[10]. Lazzari E, et al. TRIpartite motif 21 (TRIM21) differentially regulates the stability of interferon regulatory factor 5 (IRF5) isoforms. PLoS One. 2014 Aug 1;9(8):e103609. [Content Brief]

[11]. Chin W J. Functional and mechanistic studies on engineered antibodies that engage TRIM21[J]. 2020.

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TRIM21 Antibody (YA658) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane2(20μg), MOLT-4 (lane3(20μg), HeLa (lane4(20μg) and A549 (lane5(20μg) using TRIM21 Antibody (HY-P80921). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • TRIM21 Antibody (YA658) Product Image
    1/16
  • Experimental Validation Results for TRIM21 Antibody (YA658)
  • TRIM21 Antibody (YA658) Product Image
  • Experimental Validation Results for TRIM21 Antibody (YA658)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane2(20μg), MOLT-4 (lane3(20μg), HeLa (lane4(20μg) and A549 (lane5(20μg) using TRIM21 Antibody (HY-P80921). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TRIM21 Antibody (YA658)
  • TRIM21 Antibody (YA658) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293T (lane2(20μg), MOLT-4 (lane3(20μg), HeLa (lane4(20μg) and A549 (lane5(20μg) using TRIM21 Antibody (HY-P80921). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004 ,1/10,000) was used for 1 hour at room temperature.

  • TRIM21 Antibody (YA658) Product Image WB