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CD32B Antibody (YA1615)

(Synonyms: Fc fragment of IgG; low affinity IIb; receptor (CD32); CD32; FCG2; CD32B; FCGR2; IGFR2)
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Based on 1 Customer Validation

CD32B Antibody (YA1615) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD32B.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD32B Antibody (YA1615)
  • Experimental Validation Results for CD32B Antibody (YA1615)
  • CD32B Antibody (YA1615) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

CD32B Antibody (YA1615) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD32B.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 44 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 34 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human CD32B aa294-310.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103968

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD32B Antibody (YA1615)
    Western blot analysis was performed on extracts from Daudi (lane 1, 15 μg), Raji (lane 2, 15 μg), and HL-60 (lane 3, 15 μg) using CD32B Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CD32B Antibody (YA1615)
    Western blot analysis of extracts from C6 (lane 2(20μg), C6 (lane 3(40μg), using CD32B Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    CD32B is a receptor for the Fc region of immunoglobulin G complexes or aggregates. A low-affinity receptor, CD32B is involved in various effector and regulatory functions, such as the phagocytosis of immune complexes and the regulation of B cell antibody production. Binding of CD32B to this receptor leads to the regulation of cellular activation previously triggered by antigen receptors (BCRs) on B cells, antigen receptors (TCRs) on T cells, or other Fc receptors. The IIB1 isoform does not mediate endocytosis or phagocytosis. The IIB2 isoform does not trigger phagocytosis.

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is the most widely distributed Fcγ receptor. It is expressed on monocytes, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B cells, platelets, and placental (endothelial cells) . It has not been detected in natural killer cells.

  • Isoforms & Post-Translational Modification

    CD32B has 5 isomers: P31994-1: 34044 Da (predicted); P31994-2: 31944 Da (predicted); P31994-3: 33450 Da (predicted); P31994-4: 33973 Da (predicted); P31994-5: 31873 Da (predicted).
    Phosphorylated by the SRC-type Tyr-kinases LYN and BLK

  • Subunit

    Interacts with INPP5D/SHIP1. Interacts with FGR. Interacts with LYN

  • SwissProt ID

    P31994

  • Gene ID
    2213 [NCBI]
  • Synonyms

    Fc fragment of IgG; low affinity IIb; receptor (CD32); CD32; FCG2; CD32B; FCGR2; IGFR2

  • Research Field

    Immunology

CD32B Antibody (YA1615) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Daudi (lane 1, 15 μg), Raji (lane 2, 15 μg), and HL-60 (lane 3, 15 μg) using CD32B Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), C6 (lane 3(40μg), using CD32B Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD32B Antibody (YA1615) Product Image
    1/16
  • Experimental Validation Results for CD32B Antibody (YA1615)
  • Experimental Validation Results for CD32B Antibody (YA1615)
  • CD32B Antibody (YA1615) Product Image
  • Experimental Validation Results for CD32B Antibody (YA1615)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Daudi (lane 1, 15 μg), Raji (lane 2, 15 μg), and HL-60 (lane 3, 15 μg) using CD32B Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for CD32B Antibody (YA1615)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), C6 (lane 3(40μg), using CD32B Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD32B Antibody (YA1615)
  • Experimental Validation Results for CD32B Antibody (YA1615)
  • CD32B Antibody (YA1615) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Daudi (lane 1, 15 μg), Raji (lane 2, 15 μg), and HL-60 (lane 3, 15 μg) using CD32B Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • CD32B Antibody (YA1615) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6 (lane 2(20μg), C6 (lane 3(40μg), using CD32B Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD32B Antibody (YA1615) Product Image WB
  • CD32B Antibody (YA1615) Product Image WB