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Nogo B Receptor Antibody (YA1414)

(Synonyms: NgBR; MRD55; CDG1AA; C6orf68; TANGO14; MGC:7199)
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Based on 1 Customer Validation

Nogo B Receptor Antibody (YA1414) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nogo B Receptor.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Nogo B Receptor Antibody (YA1414)
  • Nogo B Receptor Antibody (YA1414) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000-1:5000

Product Details

Description

Nogo B Receptor Antibody (YA1414) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nogo B Receptor.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human Nogo B receptor

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Nogo B Receptor Antibody (YA1414)
    Western blot analysis of extracts from A549 (lane 2(20μg), K562 (lane 3(20μg), 3T3 (lane 4(20μg) and Hela (lane 5(20μg) using Nogo B Receptor Antibody (HY-P81669) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Nogo B Receptor is a With DHDDS, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Both subunits contribute to enzymatic activity, i.e. condensation of multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol phosphate which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER). Synthesizes long-chain polyprenols, mostly of C95 and C100 chain length. Regulates the glycosylation and stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol. Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator[1][2][3][4][5][6].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein

  • Subunit

    The active dehydrodolichyl diphosphate synthase complex is a heterotetramer composed of a dimer of heterodimer of DHDDS and NUS1 (PubMed:19723497, PubMed:25066056, PubMed:28842490, PubMed:32817466, PubMed:33077723, PubMed:35584224). Interacts with NPC2 (PubMed:21572394)

  • SwissProt ID

    Q96E22

  • Gene ID
    116150 [NCBI]
  • Synonyms

    NgBR; MRD55; CDG1AA; C6orf68; TANGO14; MGC:7199

  • Research Field

    -

Nogo B Receptor Antibody (YA1414) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2(20μg), K562 (lane 3(20μg), 3T3 (lane 4(20μg) and Hela (lane 5(20μg) using Nogo B Receptor Antibody (HY-P81669) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Nogo B Receptor Antibody (YA1414) Product Image
    1/16
  • Experimental Validation Results for Nogo B Receptor Antibody (YA1414)
  • Nogo B Receptor Antibody (YA1414) Product Image
  • Experimental Validation Results for Nogo B Receptor Antibody (YA1414)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2(20μg), K562 (lane 3(20μg), 3T3 (lane 4(20μg) and Hela (lane 5(20μg) using Nogo B Receptor Antibody (HY-P81669) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Nogo B Receptor Antibody (YA1414)
  • Nogo B Receptor Antibody (YA1414) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane 2(20μg), K562 (lane 3(20μg), 3T3 (lane 4(20μg) and Hela (lane 5(20μg) using Nogo B Receptor Antibody (HY-P81669) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Nogo B Receptor Antibody (YA1414) Product Image WB